Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313385215> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W4313385215 endingPage "67.7" @default.
- W4313385215 startingPage "67.7" @default.
- W4313385215 abstract "Abstract Background Matrix metalloproteinase (MMP)-9 is known to degrade the extracellular matrix and increased MMP-9 levels are related with the pathogenesis of many inflammatory conditions including obesity. Pam3CSK4 is a synthetic triacylated lipopeptide (LP) which is a potent activator of immune cells and induces cytokine production. However, it is unclear whether Pam3CSK4 is able to induce MMP-9 expression in monocytic cells. We, therefore, determined MMP-9 production by Pam3CSK4-treated THP-1 cells and also investigated the signal transduction pathway(s) involved. Methods MMP-9 expression was determined by real-time qPCR and ELISA and MMP-9 activity was assessed by zymography. THP-1 cells, THP1-XBlue™ cells, THP1-XBlue™-defMyD cells, anti-TLR2 mAb and selective pharmacological inhibitors were used to study signaling pathways involved. Phospho and total proteins were detected by western blotting. Results Pam3CSK4 induced MMP-9 expression (P<0.05) at both mRNA and protein levels in human monocytic THP-1 cells. Increased NF-κB/AP-1 activity was detected in Pam3CSK4-treated THP-1 cells and MMP-9 production in these cells was significantly suppressed by pre-treatment with anti-TLR2 neutralizing antibody or by inhibition of clathrin-dependent endocytosis. Also, MyD88−/− THP-1 cells did not express MMP-9 following treatment with Pam3CSK4. Inhibition of JNK, MEK/ERK, p38 MAPK and NF-κB significantly suppressed MMP-9 gene expression (P<0.05). Conclusion Pam3CSK4 induces MMP-9 production in THP-1 cells through the TLR-2/MyD88-dependent mechanism involving ERK, JNK, p38 MAPK and NF-κB/AP-1 activation." @default.
- W4313385215 created "2023-01-06" @default.
- W4313385215 creator A5005416741 @default.
- W4313385215 creator A5043593241 @default.
- W4313385215 creator A5059202198 @default.
- W4313385215 creator A5086374359 @default.
- W4313385215 date "2017-05-01" @default.
- W4313385215 modified "2023-09-27" @default.
- W4313385215 title "Pam3CSK4 induces MMP-9 expression in human monocytic THP-1 cells" @default.
- W4313385215 doi "https://doi.org/10.4049/jimmunol.198.supp.67.7" @default.
- W4313385215 hasPublicationYear "2017" @default.
- W4313385215 type Work @default.
- W4313385215 citedByCount "0" @default.
- W4313385215 crossrefType "journal-article" @default.
- W4313385215 hasAuthorship W4313385215A5005416741 @default.
- W4313385215 hasAuthorship W4313385215A5043593241 @default.
- W4313385215 hasAuthorship W4313385215A5059202198 @default.
- W4313385215 hasAuthorship W4313385215A5086374359 @default.
- W4313385215 hasConcept C109523444 @default.
- W4313385215 hasConcept C153911025 @default.
- W4313385215 hasConcept C185592680 @default.
- W4313385215 hasConcept C203014093 @default.
- W4313385215 hasConcept C204258353 @default.
- W4313385215 hasConcept C2776070231 @default.
- W4313385215 hasConcept C2778690821 @default.
- W4313385215 hasConcept C2781236682 @default.
- W4313385215 hasConcept C51551487 @default.
- W4313385215 hasConcept C54355233 @default.
- W4313385215 hasConcept C55493867 @default.
- W4313385215 hasConcept C57074206 @default.
- W4313385215 hasConcept C62478195 @default.
- W4313385215 hasConcept C81885089 @default.
- W4313385215 hasConcept C86803240 @default.
- W4313385215 hasConcept C95444343 @default.
- W4313385215 hasConceptScore W4313385215C109523444 @default.
- W4313385215 hasConceptScore W4313385215C153911025 @default.
- W4313385215 hasConceptScore W4313385215C185592680 @default.
- W4313385215 hasConceptScore W4313385215C203014093 @default.
- W4313385215 hasConceptScore W4313385215C204258353 @default.
- W4313385215 hasConceptScore W4313385215C2776070231 @default.
- W4313385215 hasConceptScore W4313385215C2778690821 @default.
- W4313385215 hasConceptScore W4313385215C2781236682 @default.
- W4313385215 hasConceptScore W4313385215C51551487 @default.
- W4313385215 hasConceptScore W4313385215C54355233 @default.
- W4313385215 hasConceptScore W4313385215C55493867 @default.
- W4313385215 hasConceptScore W4313385215C57074206 @default.
- W4313385215 hasConceptScore W4313385215C62478195 @default.
- W4313385215 hasConceptScore W4313385215C81885089 @default.
- W4313385215 hasConceptScore W4313385215C86803240 @default.
- W4313385215 hasConceptScore W4313385215C95444343 @default.
- W4313385215 hasIssue "1_Supplement" @default.
- W4313385215 hasLocation W43133852151 @default.
- W4313385215 hasOpenAccess W4313385215 @default.
- W4313385215 hasPrimaryLocation W43133852151 @default.
- W4313385215 hasRelatedWork W2027244603 @default.
- W4313385215 hasRelatedWork W2046649601 @default.
- W4313385215 hasRelatedWork W2086513193 @default.
- W4313385215 hasRelatedWork W2144058939 @default.
- W4313385215 hasRelatedWork W2394862083 @default.
- W4313385215 hasRelatedWork W2607359603 @default.
- W4313385215 hasRelatedWork W2889211895 @default.
- W4313385215 hasRelatedWork W3035328115 @default.
- W4313385215 hasRelatedWork W4313385215 @default.
- W4313385215 hasRelatedWork W4317436685 @default.
- W4313385215 hasVolume "198" @default.
- W4313385215 isParatext "false" @default.
- W4313385215 isRetracted "false" @default.
- W4313385215 workType "article" @default.