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- W4313385283 abstract "Abstract While the process of antibody generation in response to pathogens and immunization is well understood, the normal antibody repertoire needs characterization to better understand the diversity of the antibody response. The antibody molecule is generated from multiple gene segments; variable (V), diversity (D), and joining (J). These segments rearrange (V, D, and J in the heavy chain, V and J in the light chain) and combine with a constant region, which determines the effector function within in the host. The V, D, and J gene segments are combined to form the complementary determining region 3 (CDR3), providing much of the variability within the B cell receptor repertoire. To address heavy chain rearrangement in the unstimulated antibody repertoire, we pooled and sequenced the total splenic RNA from three groups of normal C57BL/6J. V, D, J, and constant region usage and CDR3 formation were analyzed. We developed a bioinformatic workflow to analyze the most common usage and combinations of the V, D, and J gene segments and their pairings with constant regions. We examined length and composition of CDR3 along with the ontology of the observed antibodies. The preliminary screen identified over 33,000 sequences as possible antibodies; 5,743 were hypothesized to be productive sequences. The largest V-gene family represented was V1, followed by V2 and V6. The average CDR3 length was 11 amino acids and we identified a number of high-occurrence CDR3 amino acid sequences. These data provide a glimpse of the normal C57BL/6J heavy chain response and validate the bioinformatics needed for our future immunization studies. Supported by NASA grants NNX13AN34G and NNX15AB45G and NIH grant GM103418 and the Molecular Biology Core supported by CVM-KSU." @default.
- W4313385283 created "2023-01-06" @default.
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- W4313385283 date "2016-05-01" @default.
- W4313385283 modified "2023-09-27" @default.
- W4313385283 title "Characterization of the Normal Mouse IGH Antibody Repertoire" @default.
- W4313385283 doi "https://doi.org/10.4049/jimmunol.196.supp.64.7" @default.
- W4313385283 hasPublicationYear "2016" @default.
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