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- W4313388210 abstract "Abstract Cytotoxic T lymphocytes (CTL) lyse target cells through the release of cytolytic granules and cell surface expression of Fas ligand (FasL). We found that there is prestored FasL pool in CTL, which underwent rapid TCR-regulated cell surface expression upon target cell engagement. Current models suggest that FasL is stored in cytolytic granules and that FasL cell surface expression would be subject to the same control as degranulation. The aim of the current study is to investigate if degranulation of cytolytic granules and FasL expression pathways of CTL are controlled by differential mechanisms. FasL cell surface expression and degranulation, as measured by CD107a cell surface expression in CTLs were compared by flow cytometry under different conditions. FasL underwent an immediate and transient cell surface delivery following either TCR stimulation by anti-CD3 antibody crosslinking, or phorbol ester treatment, conditions that do not permit degranulation. Furthermore, treatment with pharmacological reagents indicated that the preformed FasL cell surface transport is PI3-kinase (PI3K)-, calcineurin- and microtubule-independent, which are all indispensable for degranulation. Finally, stored FasL resides in vesicles distinct from cytolytic granules as assessed by confocal microscopy and subcellular fractionation. Our data clearly show that CTL degranulation and FasL lytic mechanisms are fully independent with respect to regulation and localization. This work was funded by the Canadian Institutes of Health Research (CIHR)." @default.
- W4313388210 created "2023-01-06" @default.
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- W4313388210 date "2007-04-01" @default.
- W4313388210 modified "2023-09-27" @default.
- W4313388210 title "Cytotoxic T lymphocytes express intracellular stores of FasL via mechanisms distinct from cytolytic granule exocytosis (B16)" @default.
- W4313388210 doi "https://doi.org/10.4049/jimmunol.178.supp.b16" @default.
- W4313388210 hasPublicationYear "2007" @default.
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