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• W4313397570 abstract "Improving the detection reliability of biosensors is essential but challenging. The dual-signal self-checking function is useful for the development of reliable biosensors; however, it inevitably increases the complexity of the sensing system. Thus, biosensors with intrinsic self-checking functions that do not require additional chemicals, specific sensor assemblies, and/or instruments are highly desirable. Herein, integrating the ability of fluorescence detection and photodegradation by carbon nitride nanosheets (CNNS), a biosensor with a self-checking function for sensitive telomerase activity detection was constructed. Acriflavine (AF) was screened from various dyes to form the most effective CNNS-AF donor-acceptor (D-A) interfaces by experiments and density functional theory (DFT) calculations, considering spectral match, electrostatic interaction, and non-covalent stacking. As a result, the proposed fluorescence biosensor not only showed highly sensitive detection of telomerase activity but also had an internal self-checking function by photodegradation of AF via continuous excitation and measurement of recovered fluorescence. This study sheds light on CNNS-based D-A interfaces with synergistic fluorescence detection and photocatalysis-induced self-checking properties for future clinical diagnosis with high sensitivity and reliability." @default.
• W4313397570 created "2023-01-06" @default.
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• W4313397570 date "2023-03-01" @default.
• W4313397570 modified "2023-10-18" @default.
• W4313397570 title "Multifunctional semiconducting carbon nitrides enabling sequential fluorescent sensing of telomerase activity and internal self-checking" @default.
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• W4313397570 doi "https://doi.org/10.1016/j.snb.2022.133170" @default.
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