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• W4313398068 startingPage "122293" @default.
• W4313398068 abstract "Aromatic amino acids play an extremely important role in life activities and participate in many biological processes. Their concentration levels are associated with a variety of diseases, such as phenylketonuria and colorectal cancer. Therefore, the quantification of aromatic amino acids is an important task. In the present work, a novel and rapid three-way analytical method was proposed to detect the levels of aromatic amino acids in prostate cancer cells (PC3 cells) and Dulbecco's modified minimal essential medium (DMEM cell culture), by using the affordable ultraviolet–visible spectrophotometer. First, spectrum-pH second-order data were designed per sample; Second, properties of the resulted spectrum-pH-sample three-way data were investigated by utilizing the parallel factor analysis (PARAFAC), alternating trilinear decomposition (ATLD), and constrained alternating trilinear decomposition (CATLD) algorithms, and a flexible scanning approach for determining the constraint parameters of CATLD was proposed; Third, a three-way calibration method based on the CATLD algorithm with the proposed scanning approach was developed for interference-free quantification of aromatic amino acids in these systems. The average relative predictive errors of validation (ARPEV) for phenylalanine, tyrosine, and tryptophan were 1.4%, 3.0%, and 0.7% in prostate cancer cells, and ARPEV for phenylalanine, tyrosine, and tryptophan were 4.1%, 1.2%, and 0.7% in DMEM cell culture. The predicted contents of tyrosine and tryptophan in DMEM cell culture were 64.2 ± 2.9 μg mL−1, 5.6 ± 0.3 μg mL−1, there are no significant differences in the concentrations between the developed analytical method and high performance liquid chromatography method. The proposed spectrum-pH-sample three-way calibration method based on CATLD algorithm can provide an interesting analytical strategy with high selectivity and accuracy for ultraviolet–visible spectrophotometer." @default.
• W4313398068 created "2023-01-06" @default.
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• W4313398068 date "2023-04-01" @default.
• W4313398068 modified "2023-10-13" @default.
• W4313398068 title "Interference-free quantitation of aromatic amino acids in two complex systems by three-way calibration with ultraviolet–visible spectrophotometer: Exploration of trilinear decomposition of spectrum-pH data" @default.
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• W4313398068 doi "https://doi.org/10.1016/j.saa.2022.122293" @default.
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• W4313398068 hasPublicationYear "2023" @default.
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