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- W4313398464 abstract "Mediatorless enzymatic biosensors based on direct electron transfer show promise due to their high stability and remarkable electrocatalytical performance. In this study, the direct electrochemistry of a glassy carbon electrode modified with hemoglobin (Hb) embedded in a hybrid layer of self-assembling EFK8 peptide and single-walled carbon nanotubes (SWNTs) was investigated. Electrochemical studies in 0.1 M phosphate buffer (PB) revealed that the immobilized Hb did not denature and retained its bio-catalytic activity. The resulting biosensor was used to detect and measure the hydrogen peroxide (H2O2) concentration in 0.1 M PB (pH 7.0), yielding a linear detection range from 20 to 960 µM and a detection limit of 7.54 µM (S/N = 3). The EFK8-SWNT hybrid layer shows promise as a biocompatible layer for simple non-covalent enzyme immobilization as the basis for future mediatorless enzymatic biosensors." @default.
- W4313398464 created "2023-01-06" @default.
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- W4313398464 date "2023-03-01" @default.
- W4313398464 modified "2023-09-25" @default.
- W4313398464 title "Direct electrochemistry of hemoglobin/peptide-carbon nanotube modified electrode for hydrogen peroxide biosensing" @default.
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- W4313398464 doi "https://doi.org/10.1016/j.matlet.2022.133799" @default.
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