FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313398516> ?p ?o ?g. }

• W4313398516 endingPage "116115" @default.
• W4313398516 startingPage "116115" @default.
• W4313398516 abstract "Berberis lycium Royle, a member of the Berberidaceae family, is a high-value medicinal plant with a documented history of usage in traditional medicine and has demonstrated significant therapeutic results among local populations throughout the globe. It is used traditionally in many parts of Pakistan to treat diarrhea, abdominal spasms, coughs, and chest problems. To investigate the antispasmodic, bronchodilator, and antidiarrheal effects of B. lycium and its possible underlying mechanisms through in silico, in vitro, and in vivo studies. LC ESI–MS/MS analysis was used to identify bioactive components within the hydromethanolic extract of B. lycium. In silico studies, including network pharmacology and molecular docking, were utilized to investigate the antispasmodic and bronchodilator properties of the extract's bioactive components. In vitro pharmacological studies were conducted using isolated rabbit jejunum, trachea, urinary bladder, and rat ileum preparations. In vivo antidiarrheal activities were conducted in mice, including castor oil-induced diarrhea, intestinal transit, and castor oil-induced enteropooling. The LC ESI–MS/MS analysis of the hydromethanolic extract of B. lycium identified 38 bioactive compounds. Network pharmacology study demonstrated that the mechanism of BLR for the treatment of diarrhea might involve IL1B, TLR4, PIK3R1, TNF, PTPRC, IL2, PIK3CD, and ABCB1, whereas, for respiratory ailments, it may involve PIK3CG, TRPV1, STAT3, ICAM1, ACE, PTGER2, PTGS2, TNF, MMP9, NOS2, IL2, CCR5, HRH1, and VDR. Molecular docking research revealed that chlorogenic acid, epigallocatechin, isorhamnetin, quinic acid, gallic acid, camptothecin, formononetin-7-O-glucoside, velutin, caffeic acid, and (S)-luteanine exhibited a higher docking score than dicyclomine with validated proteins of smooth muscle contractions such as CACB2_HUMAN, ACM3_HUMAN, MYLK_HUMAN, and PLCG1_HUMAN. In vitro investigations demonstrated that Blr.Cr, Blr.EtOAc, and Blr.Aq relaxed spontaneously contracting jejunum preparations; carbachol (1 μM)-induced and K+ (80 mM)-induced jejunum, trachea, and urinary bladder contractions in a concentration-dependent manner, similar to dicyclomine. Moreover, Blr.Cr, Blr.EtOAc, and Blr.Aq exhibited a rightward shift in Ca+2 and carbachol cumulative response curves, similar to dicyclomine, demonstrating the coexistence of antimuscarinic and Ca+2 antagonistic mechanisms due to the presence of alkaloids and flavonoids. In vivo antidiarrheal activities showed that the hydromethanolic extract was significantly effective against castor oil-induced diarrhea and castor oil-induced enteropooling, similar to loperamide, and charcoal meal intestinal transit, similar to atropine, in mice at doses of 50, 100, and 200 mg/kg body weight, which supports its traditional use in diarrhea. The dual blocking mechanism of muscarinic receptors and Ca+2 channels behind the smooth muscle relaxing activity reveals the therapeutic relevance of B. lycium in diarrhea, abdominal spasms, coughs, and chest problems." @default.
• W4313398516 created "2023-01-06" @default.
• W4313398516 creator A5038447269 @default.
• W4313398516 creator A5051643343 @default.
• W4313398516 date "2023-04-01" @default.
• W4313398516 modified "2023-09-29" @default.
• W4313398516 title "Investigations of plausible pharmacodynamics supporting the antispasmodic, bronchodilator, and antidiarrheal activities of Berberis lycium Royle. Via in silico, in vitro, and in vivo studies" @default.
• W4313398516 cites W1940329381 @default.
• W4313398516 cites W1966614349 @default.
• W4313398516 cites W1974041415 @default.
• W4313398516 cites W1977367528 @default.
• W4313398516 cites W1998648768 @default.
• W4313398516 cites W2004319004 @default.
• W4313398516 cites W2015451029 @default.
• W4313398516 cites W2023812090 @default.
• W4313398516 cites W2034865244 @default.
• W4313398516 cites W2040704062 @default.
• W4313398516 cites W2044508815 @default.
• W4313398516 cites W2050355964 @default.
• W4313398516 cites W2063551478 @default.
• W4313398516 cites W2085651819 @default.
• W4313398516 cites W2113974411 @default.
• W4313398516 cites W2145474260 @default.
• W4313398516 cites W2189534230 @default.
• W4313398516 cites W2529052575 @default.
• W4313398516 cites W2575366726 @default.
• W4313398516 cites W2736324334 @default.
• W4313398516 cites W2765247620 @default.
• W4313398516 cites W2767837707 @default.
• W4313398516 cites W2767876708 @default.
• W4313398516 cites W2891808294 @default.
• W4313398516 cites W2899140056 @default.
• W4313398516 cites W2941140597 @default.
• W4313398516 cites W2968707146 @default.
• W4313398516 cites W2973217118 @default.
• W4313398516 cites W2981871322 @default.
• W4313398516 cites W2990892923 @default.
• W4313398516 cites W3007498213 @default.
• W4313398516 cites W3016005141 @default.
• W4313398516 cites W3084428239 @default.
• W4313398516 cites W3092345291 @default.
• W4313398516 cites W3106752643 @default.
• W4313398516 cites W3109408285 @default.
• W4313398516 cites W3120925365 @default.
• W4313398516 cites W3126029576 @default.
• W4313398516 cites W3133448983 @default.
• W4313398516 cites W3151361663 @default.
• W4313398516 cites W3152774184 @default.
• W4313398516 cites W3155545542 @default.
• W4313398516 cites W3157681352 @default.
• W4313398516 cites W3178899994 @default.
• W4313398516 cites W3185111900 @default.
• W4313398516 cites W3188859863 @default.
• W4313398516 cites W4200552891 @default.
• W4313398516 cites W4237095724 @default.
• W4313398516 cites W4280529216 @default.
• W4313398516 cites W4280550274 @default.
• W4313398516 cites W4285607153 @default.
• W4313398516 cites W4304136780 @default.
• W4313398516 doi "https://doi.org/10.1016/j.jep.2022.116115" @default.
• W4313398516 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36587881" @default.
• W4313398516 hasPublicationYear "2023" @default.
• W4313398516 type Work @default.
• W4313398516 citedByCount "0" @default.
• W4313398516 crossrefType "journal-article" @default.
• W4313398516 hasAuthorship W4313398516A5038447269 @default.
• W4313398516 hasAuthorship W4313398516A5051643343 @default.
• W4313398516 hasConcept C150903083 @default.
• W4313398516 hasConcept C185592680 @default.
• W4313398516 hasConcept C207001950 @default.
• W4313398516 hasConcept C2776798904 @default.
• W4313398516 hasConcept C556039675 @default.
• W4313398516 hasConcept C71924100 @default.
• W4313398516 hasConcept C86803240 @default.
• W4313398516 hasConcept C98274493 @default.
• W4313398516 hasConceptScore W4313398516C150903083 @default.
• W4313398516 hasConceptScore W4313398516C185592680 @default.
• W4313398516 hasConceptScore W4313398516C207001950 @default.
• W4313398516 hasConceptScore W4313398516C2776798904 @default.
• W4313398516 hasConceptScore W4313398516C556039675 @default.
• W4313398516 hasConceptScore W4313398516C71924100 @default.
• W4313398516 hasConceptScore W4313398516C86803240 @default.
• W4313398516 hasConceptScore W4313398516C98274493 @default.
• W4313398516 hasLocation W43133985161 @default.
• W4313398516 hasLocation W43133985162 @default.
• W4313398516 hasOpenAccess W4313398516 @default.
• W4313398516 hasPrimaryLocation W43133985161 @default.
• W4313398516 hasRelatedWork W1963668407 @default.
• W4313398516 hasRelatedWork W2115689931 @default.
• W4313398516 hasRelatedWork W2189096324 @default.
• W4313398516 hasRelatedWork W2464315665 @default.
• W4313398516 hasRelatedWork W2610495485 @default.
• W4313398516 hasRelatedWork W2748952813 @default.
• W4313398516 hasRelatedWork W2899084033 @default.
• W4313398516 hasRelatedWork W4372258303 @default.
• W4313398516 hasRelatedWork W2291241205 @default.
• W4313398516 hasRelatedWork W2616772487 @default.
• W4313398516 hasVolume "305" @default.

• next