FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313398767> ?p ?o ?g. }

• W4313398767 endingPage "232" @default.
• W4313398767 startingPage "214" @default.
• W4313398767 abstract "Lymphocytic choriomeningitis virus (LCMV) infects many individuals worldwide and causes severe infection in the immunosuppressant recipient, spontaneous abortion, and congenital disabilities in infants.There is no specific vaccine or therapeutics available to protect against LCMV infection; thus, there is a need to design a potential vaccine to combat the virus by developing immunity in the population. Herein, we attempted to design a potent multi-epitope vaccine for LCMV using immunoinformatics methods.The whole proteome of the virus was screened and mapped to extract immunodominant B-cell and T-cell epitopes which were fused with appropriate linkers (EAAAK, GGGS, AAY, GPGPG, and AAY), PADRE sequence (13aa) and an adjuvant (50 S ribosomal protein L7/L12) to formulate a multi-epitope vaccine ensemble. Codon adaptation and in silico cloning of the constructed vaccine were carried out using bioinformatics tools. The secondary and tertiary structure of the vaccine construct was predicted and refined. The physicochemical profile of the designed vaccine was analyzed, and the multi-epitope vaccine's potential to bind Toll-like receptors (TLR2 and TLR4) was evaluated through molecular docking and molecular dynamics simulations. Computational immune simulation of the designed vaccine antigen was performed using the C-ImmSim server.The designed multi-epitope-based vaccine (613 aa) comprised 26 immunodominant (six B-cell, nine cytotoxic T lymphocytes, and 11 helper T lymphocytes) epitopes and is predicted antigenic, non-toxic, non-allergen, soluble, and topographically accessible with a suitable physicochemical profile. The designed vaccine is expected to cover a broad worldwide population (96.35 %) and stimulate a robust adaptive immune response against the virus upon administration. In silico cloning of the constructed vaccine in PET28a (+) vector ensured its optimal expression in the Escherichia coli system. Molecular docking, molecular dynamics simulation, and binding free energy estimation collectively support the stability and energetically favourable interaction of the modeled vaccine-TLR2/4 complexes.The designed multi-epitope vaccine in the present study could serve as a potential vaccine candidate to protect against LMCV infection; however, the experimental validation and safety testing of the vaccine is warranted to validate the study's outcomes." @default.
• W4313398767 created "2023-01-06" @default.
• W4313398767 creator A5001178856 @default.
• W4313398767 creator A5001710105 @default.
• W4313398767 creator A5032226045 @default.
• W4313398767 creator A5059173207 @default.
• W4313398767 creator A5076140757 @default.
• W4313398767 creator A5078772483 @default.
• W4313398767 creator A5079620679 @default.
• W4313398767 creator A5084747381 @default.
• W4313398767 creator A5086756289 @default.
• W4313398767 creator A5091778180 @default.
• W4313398767 date "2023-02-01" @default.
• W4313398767 modified "2023-09-30" @default.
• W4313398767 title "Employing an immunoinformatics approach revealed potent multi-epitope based subunit vaccine for lymphocytic choriomeningitis virus" @default.
• W4313398767 cites W1503364994 @default.
• W4313398767 cites W1606410455 @default.
• W4313398767 cites W1771326673 @default.
• W4313398767 cites W1968361401 @default.
• W4313398767 cites W1970847296 @default.
• W4313398767 cites W1973980688 @default.
• W4313398767 cites W1995652170 @default.
• W4313398767 cites W2002411728 @default.
• W4313398767 cites W2005846325 @default.
• W4313398767 cites W2012625502 @default.
• W4313398767 cites W2014274241 @default.
• W4313398767 cites W2015176909 @default.
• W4313398767 cites W2020397172 @default.
• W4313398767 cites W2020719323 @default.
• W4313398767 cites W2035240910 @default.
• W4313398767 cites W2043944957 @default.
• W4313398767 cites W2054086585 @default.
• W4313398767 cites W2067174909 @default.
• W4313398767 cites W2071380243 @default.
• W4313398767 cites W2074512572 @default.
• W4313398767 cites W2077591905 @default.
• W4313398767 cites W2098583514 @default.
• W4313398767 cites W2100561975 @default.
• W4313398767 cites W2102367710 @default.
• W4313398767 cites W2102993309 @default.
• W4313398767 cites W2105422109 @default.
• W4313398767 cites W2118817344 @default.
• W4313398767 cites W2120467372 @default.
• W4313398767 cites W2123325078 @default.
• W4313398767 cites W2127635589 @default.
• W4313398767 cites W2128672595 @default.
• W4313398767 cites W2133369735 @default.
• W4313398767 cites W2133458779 @default.
• W4313398767 cites W2136126826 @default.
• W4313398767 cites W2142529984 @default.
• W4313398767 cites W2142868265 @default.
• W4313398767 cites W2150114245 @default.
• W4313398767 cites W2151932306 @default.
• W4313398767 cites W2155201844 @default.
• W4313398767 cites W2159767366 @default.
• W4313398767 cites W2164270482 @default.
• W4313398767 cites W2168036974 @default.
• W4313398767 cites W2287500273 @default.
• W4313398767 cites W2290371181 @default.
• W4313398767 cites W2313116527 @default.
• W4313398767 cites W2332712348 @default.
• W4313398767 cites W2342838938 @default.
• W4313398767 cites W2419493891 @default.
• W4313398767 cites W2515457741 @default.
• W4313398767 cites W2585451190 @default.
• W4313398767 cites W2586246990 @default.
• W4313398767 cites W2588895177 @default.
• W4313398767 cites W2607286972 @default.
• W4313398767 cites W2611314002 @default.
• W4313398767 cites W2620185010 @default.
• W4313398767 cites W2730472814 @default.
• W4313398767 cites W2748834817 @default.
• W4313398767 cites W2751476102 @default.
• W4313398767 cites W2800076205 @default.
• W4313398767 cites W2897003153 @default.
• W4313398767 cites W2922042974 @default.
• W4313398767 cites W2922503279 @default.
• W4313398767 cites W2927634357 @default.
• W4313398767 cites W2989052455 @default.
• W4313398767 cites W2999790763 @default.
• W4313398767 cites W2999919945 @default.
• W4313398767 cites W3004816968 @default.
• W4313398767 cites W3007962889 @default.
• W4313398767 cites W3016853422 @default.
• W4313398767 cites W3022046133 @default.
• W4313398767 cites W3039821298 @default.
• W4313398767 cites W3047917919 @default.
• W4313398767 cites W3047935184 @default.
• W4313398767 cites W3086109783 @default.
• W4313398767 cites W3127135885 @default.
• W4313398767 cites W3134101032 @default.
• W4313398767 cites W3163904779 @default.
• W4313398767 cites W3184818553 @default.
• W4313398767 cites W3186179742 @default.
• W4313398767 cites W3195029928 @default.
• W4313398767 cites W3204529400 @default.
• W4313398767 cites W3204868409 @default.
• W4313398767 cites W3216792204 @default.

• next