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- W4313404903 abstract "Abstract Introduction Previously, we demonstrated that maternal high fat diet (mHFD) exposure results in a unique pattern of microbial colonization in offspring, expansion of IL-17 producing group 3 innate lymphoid cells (ILC3) and increased susceptibility to a model of necrotizing enterocolitis (NEC). We hypothesized that amniotic fluid (AF) metabolites are modified by maternal diet and partially responsible for the phenotype in offspring. Methods To assess if AF metabolites in mHFD were unique from regular diet (RD) AF, we collected AF from E15 dams and performed non-targeted global mass spectroscopy. To determine if the mHFD AF is responsible for expansion of ILC3, we gavaged 5-day-old RD germ-free pups with mHFD and RD AF (from conventional dams) every other day and examined them at 2 weeks by flow cytometry for ILC3. mHFD mothers were supplemented with metabolites that were decreased in the mHFD AF and offspring examined for expansion of ILC3 and susceptibility to the LPS/PAF model of NEC. Results We identified 1069 metabolites in HFD and RD AF. Principal component analysis revealed that mHFD AF was uniquely clustered from RD AF. We specifically found decreases in indole, short chain fatty acid and furoic acid metabolites in HFD AF compared to RD AF. Gavage of germ-free neonatal pups with mHFD AF resulted in expansion of IL-17 producing ILC3 compared to RD AF at 2 weeks. Supplementation of mHFD dams prenatally with metabolites diminished in HFD AF resulted in decreased expansion of ILC3 and resistance to LPS/PAF model of NEC. Conclusion mHFD modifies metabolites present in AF and can expand ILC3 in germ-free offspring. Supplementation of deficient dietary metabolites in mHFD pregnant dams rescues offspring from susceptibility to NEC. Supported by NIH R01 1K12HD068369" @default.
- W4313404903 created "2023-01-06" @default.
- W4313404903 creator A5017844537 @default.
- W4313404903 date "2022-05-01" @default.
- W4313404903 modified "2023-09-23" @default.
- W4313404903 title "Amniotic fluid metabolites mediate maternal high fat diet expansion of group 3 innate lymphoid cells in offspring." @default.
- W4313404903 doi "https://doi.org/10.4049/jimmunol.208.supp.59.12" @default.
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