Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313405736> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W4313405736 endingPage "123.07" @default.
- W4313405736 startingPage "123.07" @default.
- W4313405736 abstract "Abstract In recent years, metabolism has emerged as a key driver of immune cell function in both healthy and disease settings. Indeed, it has been shown that metabolic reprogramming is a determinant of T cell phenotype and fate. Moreover, reprogramming of the metabolic pathways of T cells can be used as a strategy to improve the antitumor efficacy of adoptive T cell therapies. The Agilent Seahorse XF T cell Metabolic Profiling (XF TCMP) kit is a new solution that, combined with the Seahorse XF analyzer, allows for the simultaneous measurement of basal metabolic demand, metabolic poise and respiratory capacity in T cells. These parameters have been linked with optimal function and improved persistence of T cell therapies. The assay uses an improved uncoupler (BAM15) for more robust measurements of T cell bioenergetic capacity with less optimization than other uncouplers used. The XF TCMP kit was used to evaluate the metabolic phenotype of human T cells expanded in different nutrient conditions. T cells expanded in different cell culture media resulted in populations with different metabolic profiles. These metabolic profiles correlated with different functional markers, supporting the link between metabolism and function. The XF TCMP kit was also used to assess metabolic poise and bioenergetic capacity under nutrient-restriction, allowing us to better understand fuel requirements after expansion. Together, the data show that the XF TCMP kit provides a complete metabolic assessment of T cells in real-time. The assay can be used to develop improved anti-tumor T cell therapies by evaluating strategies that improve the persistence of infused cells or assess how T cells sustain metabolic fitness in nutrient restricted environments to maintain function." @default.
- W4313405736 created "2023-01-06" @default.
- W4313405736 creator A5055740278 @default.
- W4313405736 creator A5064329985 @default.
- W4313405736 creator A5067400859 @default.
- W4313405736 date "2022-05-01" @default.
- W4313405736 modified "2023-09-27" @default.
- W4313405736 title "A Novel Solution to Better Understand the Link Between T Cell Metabolism and Function" @default.
- W4313405736 doi "https://doi.org/10.4049/jimmunol.208.supp.123.07" @default.
- W4313405736 hasPublicationYear "2022" @default.
- W4313405736 type Work @default.
- W4313405736 citedByCount "0" @default.
- W4313405736 crossrefType "journal-article" @default.
- W4313405736 hasAuthorship W4313405736A5055740278 @default.
- W4313405736 hasAuthorship W4313405736A5064329985 @default.
- W4313405736 hasAuthorship W4313405736A5067400859 @default.
- W4313405736 hasConcept C100206155 @default.
- W4313405736 hasConcept C104317684 @default.
- W4313405736 hasConcept C127716648 @default.
- W4313405736 hasConcept C14036430 @default.
- W4313405736 hasConcept C1491633281 @default.
- W4313405736 hasConcept C192989942 @default.
- W4313405736 hasConcept C203014093 @default.
- W4313405736 hasConcept C2776090121 @default.
- W4313405736 hasConcept C28859421 @default.
- W4313405736 hasConcept C55493867 @default.
- W4313405736 hasConcept C62231903 @default.
- W4313405736 hasConcept C86803240 @default.
- W4313405736 hasConcept C8891405 @default.
- W4313405736 hasConcept C95444343 @default.
- W4313405736 hasConceptScore W4313405736C100206155 @default.
- W4313405736 hasConceptScore W4313405736C104317684 @default.
- W4313405736 hasConceptScore W4313405736C127716648 @default.
- W4313405736 hasConceptScore W4313405736C14036430 @default.
- W4313405736 hasConceptScore W4313405736C1491633281 @default.
- W4313405736 hasConceptScore W4313405736C192989942 @default.
- W4313405736 hasConceptScore W4313405736C203014093 @default.
- W4313405736 hasConceptScore W4313405736C2776090121 @default.
- W4313405736 hasConceptScore W4313405736C28859421 @default.
- W4313405736 hasConceptScore W4313405736C55493867 @default.
- W4313405736 hasConceptScore W4313405736C62231903 @default.
- W4313405736 hasConceptScore W4313405736C86803240 @default.
- W4313405736 hasConceptScore W4313405736C8891405 @default.
- W4313405736 hasConceptScore W4313405736C95444343 @default.
- W4313405736 hasIssue "1_Supplement" @default.
- W4313405736 hasLocation W43134057361 @default.
- W4313405736 hasOpenAccess W4313405736 @default.
- W4313405736 hasPrimaryLocation W43134057361 @default.
- W4313405736 hasRelatedWork W1648324402 @default.
- W4313405736 hasRelatedWork W1993094357 @default.
- W4313405736 hasRelatedWork W2036930304 @default.
- W4313405736 hasRelatedWork W2410854506 @default.
- W4313405736 hasRelatedWork W2614164673 @default.
- W4313405736 hasRelatedWork W3150474329 @default.
- W4313405736 hasRelatedWork W3160335747 @default.
- W4313405736 hasRelatedWork W4200034620 @default.
- W4313405736 hasRelatedWork W4297807345 @default.
- W4313405736 hasRelatedWork W4322758023 @default.
- W4313405736 hasVolume "208" @default.
- W4313405736 isParatext "false" @default.
- W4313405736 isRetracted "false" @default.
- W4313405736 workType "article" @default.