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- W4313405951 abstract "Abstract The detection of a specific analyte in a complex mixture by an immunoassay is a common procedure in many bioanalytical workflows. Immunoassays rely on the interaction between labeled antibodies and the analyte of interest. We have developed Lumit, a novel bioluminescent labeling approach allowing no-wash immunoassays amenable for high-throughput applications. Lumit immunoassay technology is based on an adaptation of NanoBiT® luciferase, a structural complementation system that consists of the Large BiT (LgBiT; 18 kDa) subunit and a small, complementary peptide, Small BiT (SmBiT; 11 amino acids), that has low affinity for LgBiT. Antibodies labeled with LgBiT or SmBiT subunits are brought into proximity due to binding to a target analyte. This facilitated complementation reconstitutes an active luciferase and generates light proportional to the amount of analyte present. This detection chemistry has several advantages over traditional ELISA workflows, including a no-transfer and no-wash protocol suitable for automation, fast detection time (30 – 90 minutes), and a broad dynamic range mitigating the need for sample dilutions. Here, we present a variety of applications of Lumit technology to detect analytes in immunology research. Examples include detection of cytokines from activated cultured immune cells using labeled primary antibodies, the detection of antibody-Fc receptor binding, the detection of protein phosphorylation using labeled secondary antibodies, and detection of the PD1: PDL1 interaction using labeled anti-tag antibodies." @default.
- W4313405951 created "2023-01-06" @default.
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- W4313405951 date "2022-05-01" @default.
- W4313405951 modified "2023-09-27" @default.
- W4313405951 title "Lumit: a novel bioluminescent, homogenous immunoassay platform for analyte detection" @default.
- W4313405951 doi "https://doi.org/10.4049/jimmunol.208.supp.173.12" @default.
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