Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313406072> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W4313406072 endingPage "112.08" @default.
- W4313406072 startingPage "112.08" @default.
- W4313406072 abstract "Abstract During a humoral immune response, B cells produce alternative immunoglobulin isotypes (IgG, IgA, IgE) through class switch recombination (CSR). Initiation of CSR requires AID-mediated deamination of switch (S) regions in the immunoglobulin heavy chain (IgH) locus. Although G-quadruplex(G4)-forming S region RNAs bind AID and localize AID to S region DNA sequences, the molecular mechanism that regulates the transition of AID binding from RNA to DNA remains uncharacterized. Highly stable G4 structures necessitate helicases to unwind the G-G hydrogen bonds, which in turn may permit the base-pairing of the S transcript to the complementary DNA sequence in the IgH locus during CSR. An S region RNA pull-down assay identified the RNA Helicase associated with AU-rich element (RHAU), a DEAH-box RNA helicase, as an S transcript binding protein. To examine the role of RHAU in CSR, we genetically deleted a floxed RHAU allele in B cells of mice using CD23-Cre. Conditional deletion of RHAU in B cells reduced CSR in vivo and in vitro to approximately 50% of wild-type controls. This data suggests that RHAU plays an important role in CSR. We hypothesize that RHAU unwinds G4 in S transcripts to facilitate the handoff of AID from G4-RNA to S region DNA during CSR. Supported by The National Cancer Institute (2U54CA132378) and The National Institute of General Medical Sciences (1SC1GM132035-01)" @default.
- W4313406072 created "2023-01-06" @default.
- W4313406072 creator A5048386453 @default.
- W4313406072 creator A5049089741 @default.
- W4313406072 creator A5060341404 @default.
- W4313406072 creator A5064251720 @default.
- W4313406072 date "2022-05-01" @default.
- W4313406072 modified "2023-09-23" @default.
- W4313406072 title "The DEAH-box helicase RHAU regulates immunoglobulin class switch recombination" @default.
- W4313406072 doi "https://doi.org/10.4049/jimmunol.208.supp.112.08" @default.
- W4313406072 hasPublicationYear "2022" @default.
- W4313406072 type Work @default.
- W4313406072 citedByCount "0" @default.
- W4313406072 crossrefType "journal-article" @default.
- W4313406072 hasAuthorship W4313406072A5048386453 @default.
- W4313406072 hasAuthorship W4313406072A5049089741 @default.
- W4313406072 hasAuthorship W4313406072A5060341404 @default.
- W4313406072 hasAuthorship W4313406072A5064251720 @default.
- W4313406072 hasConcept C104317684 @default.
- W4313406072 hasConcept C137756551 @default.
- W4313406072 hasConcept C153911025 @default.
- W4313406072 hasConcept C159654299 @default.
- W4313406072 hasConcept C161223559 @default.
- W4313406072 hasConcept C185581394 @default.
- W4313406072 hasConcept C2778453870 @default.
- W4313406072 hasConcept C54355233 @default.
- W4313406072 hasConcept C552990157 @default.
- W4313406072 hasConcept C67705224 @default.
- W4313406072 hasConcept C68592252 @default.
- W4313406072 hasConcept C86803240 @default.
- W4313406072 hasConcept C96007430 @default.
- W4313406072 hasConceptScore W4313406072C104317684 @default.
- W4313406072 hasConceptScore W4313406072C137756551 @default.
- W4313406072 hasConceptScore W4313406072C153911025 @default.
- W4313406072 hasConceptScore W4313406072C159654299 @default.
- W4313406072 hasConceptScore W4313406072C161223559 @default.
- W4313406072 hasConceptScore W4313406072C185581394 @default.
- W4313406072 hasConceptScore W4313406072C2778453870 @default.
- W4313406072 hasConceptScore W4313406072C54355233 @default.
- W4313406072 hasConceptScore W4313406072C552990157 @default.
- W4313406072 hasConceptScore W4313406072C67705224 @default.
- W4313406072 hasConceptScore W4313406072C68592252 @default.
- W4313406072 hasConceptScore W4313406072C86803240 @default.
- W4313406072 hasConceptScore W4313406072C96007430 @default.
- W4313406072 hasIssue "1_Supplement" @default.
- W4313406072 hasLocation W43134060721 @default.
- W4313406072 hasOpenAccess W4313406072 @default.
- W4313406072 hasPrimaryLocation W43134060721 @default.
- W4313406072 hasRelatedWork W10114010 @default.
- W4313406072 hasRelatedWork W1560452225 @default.
- W4313406072 hasRelatedWork W1976736675 @default.
- W4313406072 hasRelatedWork W2002473487 @default.
- W4313406072 hasRelatedWork W2007245155 @default.
- W4313406072 hasRelatedWork W2051801995 @default.
- W4313406072 hasRelatedWork W2068738382 @default.
- W4313406072 hasRelatedWork W2077034711 @default.
- W4313406072 hasRelatedWork W2111497364 @default.
- W4313406072 hasRelatedWork W2762814002 @default.
- W4313406072 hasVolume "208" @default.
- W4313406072 isParatext "false" @default.
- W4313406072 isRetracted "false" @default.
- W4313406072 workType "article" @default.