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- W4313406936 abstract "Abstract Evidence suggests there are important differences between reptile and mammalian immune strategies, yet little is known about humoral immune responses in reptilian vertebrates. Our laboratory is interested in reptile B cell development and function and our undergraduate research project involved B cell detection using flow cytometry in spleens of hatchling and adult turtles. We made use of a previously developed monoclonal antibody (HL673) that recognizes turtle light chain protein. The murine HL673 mAb was purified using a protein A affinity column and reactivity was tested by ELISA. Some of the purified antibody was biotinylated and incubated with blood and spleen samples from adult and hatchlings. Bound antibodies were detected and B cell populations identified using flow cytometry. We were able to identify B cell containing populations based on scatter and specific staining. Our results showed detection of turtle B cells using the labeled mAb in both adults and 6 week old hatchlings. Future studies will use this reagent to further investigate the distribution and function of B cells. This work was supported by NSF 1725199 and NIH 1R15AI140118 – 01." @default.
- W4313406936 created "2023-01-06" @default.
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- W4313406936 date "2022-05-01" @default.
- W4313406936 modified "2023-10-16" @default.
- W4313406936 title "Identifying B cells in hatchling and adult <i>Trachemys scripta</i>, red-eared slider turtles" @default.
- W4313406936 doi "https://doi.org/10.4049/jimmunol.208.supp.124.09" @default.
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