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- W4313407787 abstract "This chapter describes the culture of various continuous cell lines. Continuous cell lines are generated either due to specific genetic or epigenetic alterations in the genome of certain cells. The reasons for genetic alterations are the manifestation of spontaneous or induced mutations via radiations and chemical or biological agents such as viruses. In the experimental laboratory conditions, continuous cell lines can be generated by a viral infection, biochemical agents, or radiations. This chapter discusses the culture of major mammalian continuous/cancer cell lines, including breast cancer cell line (MCF-7 and MDA-MB-231 cell), ovarian cancer cell line (CHO cells), prostate cancer cell line (LNCaP and PC3 cells), cancer cell lines originating from the female cervix (HeLa cells), lung cancer cell lines (A549 and BEAS-2B cells), kidney cancer cell lines (HEK-293 and VERO cells), liver cancer cell lines (HepG2 cells), and blood cancer cell lines (JURKAT and RAW cells). While some of the abovementioned cancer cell lines are isolated directly from various cancer patients, others are experimentally generated in the laboratory. The knowledge gained from this chapter would help to understand the culture and regular maintenance of the abovementioned continuous cell lines and in the development of various anticancer agents." @default.
- W4313407787 created "2023-01-06" @default.
- W4313407787 creator A5027813989 @default.
- W4313407787 date "2023-01-01" @default.
- W4313407787 modified "2023-10-18" @default.
- W4313407787 title "Culture of Continuous Cell Lines" @default.
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- W4313407787 doi "https://doi.org/10.1007/978-981-19-1731-8_11-1" @default.
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