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• W4313407814 abstract "This chapter begins with a brief description of the mechanisms associated with maintaining immunity (protection against disease-causing pathogens) in the mammalian body. All the immunological cells whether involved in innate (inherited from parents to offspring) or acquired immunity (newly developed in response to pathogen) originated from hematopoietic stem cells (CD34+) within the red bone marrow. The immunological cells are not only present in the body fluids (e.g., blood, lymph, etc.), but also in the primary lymphoid organs such as red bone marrow and thymus as well as secondary lymphoid organs such as the spleen, lymph nodes, mucosa-, and gut-associated lymphoid organs (MALT and GALT), respectively. This chapter describes the isolation of various primary and secondary lymphoid organs such as the red bone marrow, thymus, spleen, and lymph nodes as well as different immunological cells present in these organs and body fluids such as blood. Isolation, purification, enrichment, and culture of the innate immunological cells including monocytes and tissue macrophages from various regions of a mammalian body and other immunological cells, viz., dendritic cells and natural killer (NK) cells, are discussed. In addition, isolation, purification, enrichment, and culture of acquired immunological cells, i.e., both B cells and various T cells (T helper, T cytotoxic, T regulatory), and γδ T cells, as well as isolation and culture of a natural killer like T cells (NKT), an invariant natural killer like T (iNKT) cells, and co-culture of various cells, are described. The chapter ends with a very brief note on the significance of isolation and culture of these different mammalian immunological cells." @default.
• W4313407814 created "2023-01-06" @default.
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• W4313407814 date "2023-01-01" @default.
• W4313407814 modified "2023-10-12" @default.
• W4313407814 title "Primary Culture of Immunological Cells" @default.
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• W4313407814 doi "https://doi.org/10.1007/978-981-19-1731-8_9-1" @default.
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