Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313407876> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W4313407876 endingPage "163.35" @default.
- W4313407876 startingPage "163.35" @default.
- W4313407876 abstract "Abstract Pathogenic microbes have evolved various mechanisms to evade recognition by host immune responses, which can lead to the prevalence of disease and infection. Many strains of pathogenic bacteria contain genes encoding Toll/Interleukin-1 receptor (TIR) domain-containing proteins that function through the inhibition of TLR and myeloid differentiation factor 88 (MyD88) signaling. Previous research has also revealed that prokaryotic TIR domains may regulate bacterial and host metabolic pathways by acting as NAD+ glycohydrolases (NADases). We hypothesize that this NADase activity may contribute to the role of bacterial TIRs as virulence factors. In order to test this hypothesis, we observed the effects of TIR NADase activity on the binding interactions between bacterial TIRs and host MyD88, and on the ability of bacterial TIRs to block NF-kB signaling. For this, we have identified amino acids predicted to play a role in TIR NADase activity and have expressed and purified recombinant bacterial TIR proteins that have incorporated these mutations. We then tested the NADase activity of these mutants in comparison with that of wild-type TIR proteins and TIRs with a mutation known to inhibit NADase activity. We are now using TIRs with deficiencies in NADase activity in pull-down assays to test their binding to MyD88 and utilizing RT-PCR to analyze the effects of these mutant TIRs on innate cell activation. This information will allow us to gain a deeper understanding into the relationship between NADase activity in bacterial TIRs and their virulence activity and can potentially uncover new mechanisms for pathogenic evasion of host immune defenses. Supported by grant from NIH (R25 GM119970 04)" @default.
- W4313407876 created "2023-01-06" @default.
- W4313407876 creator A5021748155 @default.
- W4313407876 creator A5039957651 @default.
- W4313407876 creator A5044396416 @default.
- W4313407876 creator A5054727819 @default.
- W4313407876 creator A5065680919 @default.
- W4313407876 creator A5074136006 @default.
- W4313407876 date "2022-05-01" @default.
- W4313407876 modified "2023-09-26" @default.
- W4313407876 title "NADase activity found in bacterial TIR proteins may aid in innate immune evasion" @default.
- W4313407876 doi "https://doi.org/10.4049/jimmunol.208.supp.163.35" @default.
- W4313407876 hasPublicationYear "2022" @default.
- W4313407876 type Work @default.
- W4313407876 citedByCount "0" @default.
- W4313407876 crossrefType "journal-article" @default.
- W4313407876 hasAuthorship W4313407876A5021748155 @default.
- W4313407876 hasAuthorship W4313407876A5039957651 @default.
- W4313407876 hasAuthorship W4313407876A5044396416 @default.
- W4313407876 hasAuthorship W4313407876A5054727819 @default.
- W4313407876 hasAuthorship W4313407876A5065680919 @default.
- W4313407876 hasAuthorship W4313407876A5074136006 @default.
- W4313407876 hasConcept C104317684 @default.
- W4313407876 hasConcept C136449434 @default.
- W4313407876 hasConcept C143065580 @default.
- W4313407876 hasConcept C2781090273 @default.
- W4313407876 hasConcept C2781251061 @default.
- W4313407876 hasConcept C54355233 @default.
- W4313407876 hasConcept C60987743 @default.
- W4313407876 hasConcept C86803240 @default.
- W4313407876 hasConcept C8891405 @default.
- W4313407876 hasConcept C89423630 @default.
- W4313407876 hasConcept C95444343 @default.
- W4313407876 hasConceptScore W4313407876C104317684 @default.
- W4313407876 hasConceptScore W4313407876C136449434 @default.
- W4313407876 hasConceptScore W4313407876C143065580 @default.
- W4313407876 hasConceptScore W4313407876C2781090273 @default.
- W4313407876 hasConceptScore W4313407876C2781251061 @default.
- W4313407876 hasConceptScore W4313407876C54355233 @default.
- W4313407876 hasConceptScore W4313407876C60987743 @default.
- W4313407876 hasConceptScore W4313407876C86803240 @default.
- W4313407876 hasConceptScore W4313407876C8891405 @default.
- W4313407876 hasConceptScore W4313407876C89423630 @default.
- W4313407876 hasConceptScore W4313407876C95444343 @default.
- W4313407876 hasIssue "1_Supplement" @default.
- W4313407876 hasLocation W43134078761 @default.
- W4313407876 hasOpenAccess W4313407876 @default.
- W4313407876 hasPrimaryLocation W43134078761 @default.
- W4313407876 hasRelatedWork W1941223470 @default.
- W4313407876 hasRelatedWork W1987775628 @default.
- W4313407876 hasRelatedWork W2018115329 @default.
- W4313407876 hasRelatedWork W2102838553 @default.
- W4313407876 hasRelatedWork W2116265362 @default.
- W4313407876 hasRelatedWork W2132479441 @default.
- W4313407876 hasRelatedWork W2561444898 @default.
- W4313407876 hasRelatedWork W2855659600 @default.
- W4313407876 hasRelatedWork W3141517888 @default.
- W4313407876 hasRelatedWork W4248881322 @default.
- W4313407876 hasVolume "208" @default.
- W4313407876 isParatext "false" @default.
- W4313407876 isRetracted "false" @default.
- W4313407876 workType "article" @default.