Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313408051> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W4313408051 endingPage "164.06" @default.
- W4313408051 startingPage "164.06" @default.
- W4313408051 abstract "Abstract Pattern recognition receptors (PRRs) are nearly ubiquitous in both immune and non-immune mammalian cells. PRRs detect pathogen invasion as well as internal damage, inducing the secretion of pro-inflammatory molecules and cell death. Members of the PRR subset that detect intracellular DNA are termed DNA sensors. We examined DNA sensing in non-immune cells and their equivalent tissues using electroporation. This physical delivery method mitigates the need for viral or chemical agents that could confound the source of PRR activation. We used RT-qPCR and/or RNA-Seq to quantify mRNAs encoding DNA sensors and their signaling pathways and western blots, ELISAs and bead arrays to quantify related proteins. Several tumor cell types and non-tumor cells, including keratinocytes, fibroblasts, and myoblasts, expressed different DNA sensors at baseline and responded to backbone plasmid DNA delivery with individual DNA sensor signatures. Some similarities were observed among all these cell types, including upregulation of the DNA sensors ZBP1, DDX60, and Ifi204, the mouse analog of human Ifi16. Each cell type secreted a range of pro-inflammatory molecules including Type I interferon. DNA electroporation to B16-F10 melanomas, skin, and skeletal muscle induced unique cytokine and chemokine signatures, paralleling cellular signatures. ZBP1, DDX60, and Ifi204 were upregulated in skin and muscle; however, no significant regulation was observed in tumors. In muscle particularly, this may be due to the homogeneity of the tissue; tumors are inherently heterogeneous, producing an assortment of individual cellular responses. Supported by NIH grant R01CA196796." @default.
- W4313408051 created "2023-01-06" @default.
- W4313408051 creator A5002399442 @default.
- W4313408051 creator A5010262841 @default.
- W4313408051 creator A5021065744 @default.
- W4313408051 creator A5041997129 @default.
- W4313408051 creator A5048547302 @default.
- W4313408051 creator A5056214204 @default.
- W4313408051 creator A5061936570 @default.
- W4313408051 creator A5070257255 @default.
- W4313408051 creator A5086406281 @default.
- W4313408051 date "2022-05-01" @default.
- W4313408051 modified "2023-10-18" @default.
- W4313408051 title "Comparison of DNA sensing by non-immune cells and tissues" @default.
- W4313408051 doi "https://doi.org/10.4049/jimmunol.208.supp.164.06" @default.
- W4313408051 hasPublicationYear "2022" @default.
- W4313408051 type Work @default.
- W4313408051 citedByCount "0" @default.
- W4313408051 crossrefType "journal-article" @default.
- W4313408051 hasAuthorship W4313408051A5002399442 @default.
- W4313408051 hasAuthorship W4313408051A5010262841 @default.
- W4313408051 hasAuthorship W4313408051A5021065744 @default.
- W4313408051 hasAuthorship W4313408051A5041997129 @default.
- W4313408051 hasAuthorship W4313408051A5048547302 @default.
- W4313408051 hasAuthorship W4313408051A5056214204 @default.
- W4313408051 hasAuthorship W4313408051A5061936570 @default.
- W4313408051 hasAuthorship W4313408051A5070257255 @default.
- W4313408051 hasAuthorship W4313408051A5086406281 @default.
- W4313408051 hasConcept C104317684 @default.
- W4313408051 hasConcept C13373296 @default.
- W4313408051 hasConcept C143425029 @default.
- W4313408051 hasConcept C1491633281 @default.
- W4313408051 hasConcept C153911025 @default.
- W4313408051 hasConcept C186852380 @default.
- W4313408051 hasConcept C189014844 @default.
- W4313408051 hasConcept C203014093 @default.
- W4313408051 hasConcept C2776178377 @default.
- W4313408051 hasConcept C54009773 @default.
- W4313408051 hasConcept C54355233 @default.
- W4313408051 hasConcept C552990157 @default.
- W4313408051 hasConcept C79879829 @default.
- W4313408051 hasConcept C81885089 @default.
- W4313408051 hasConcept C86803240 @default.
- W4313408051 hasConcept C8891405 @default.
- W4313408051 hasConcept C95444343 @default.
- W4313408051 hasConceptScore W4313408051C104317684 @default.
- W4313408051 hasConceptScore W4313408051C13373296 @default.
- W4313408051 hasConceptScore W4313408051C143425029 @default.
- W4313408051 hasConceptScore W4313408051C1491633281 @default.
- W4313408051 hasConceptScore W4313408051C153911025 @default.
- W4313408051 hasConceptScore W4313408051C186852380 @default.
- W4313408051 hasConceptScore W4313408051C189014844 @default.
- W4313408051 hasConceptScore W4313408051C203014093 @default.
- W4313408051 hasConceptScore W4313408051C2776178377 @default.
- W4313408051 hasConceptScore W4313408051C54009773 @default.
- W4313408051 hasConceptScore W4313408051C54355233 @default.
- W4313408051 hasConceptScore W4313408051C552990157 @default.
- W4313408051 hasConceptScore W4313408051C79879829 @default.
- W4313408051 hasConceptScore W4313408051C81885089 @default.
- W4313408051 hasConceptScore W4313408051C86803240 @default.
- W4313408051 hasConceptScore W4313408051C8891405 @default.
- W4313408051 hasConceptScore W4313408051C95444343 @default.
- W4313408051 hasIssue "1_Supplement" @default.
- W4313408051 hasLocation W43134080511 @default.
- W4313408051 hasOpenAccess W4313408051 @default.
- W4313408051 hasPrimaryLocation W43134080511 @default.
- W4313408051 hasRelatedWork W1548891476 @default.
- W4313408051 hasRelatedWork W1970605675 @default.
- W4313408051 hasRelatedWork W1979006359 @default.
- W4313408051 hasRelatedWork W2021032653 @default.
- W4313408051 hasRelatedWork W2141284323 @default.
- W4313408051 hasRelatedWork W2371788052 @default.
- W4313408051 hasRelatedWork W2381516815 @default.
- W4313408051 hasRelatedWork W2385919261 @default.
- W4313408051 hasRelatedWork W2388976704 @default.
- W4313408051 hasRelatedWork W2392518530 @default.
- W4313408051 hasVolume "208" @default.
- W4313408051 isParatext "false" @default.
- W4313408051 isRetracted "false" @default.
- W4313408051 workType "article" @default.