FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313410109> ?p ?o ?g. }

• W4313410109 endingPage "17" @default.
• W4313410109 startingPage "1" @default.
• W4313410109 abstract "This study was aimed to explore the mechanism of Sishen Wan (SSW) in treating ulcerative colitis (UC) in a rat model of spleen-kidney yang deficiency pattern by regulating gut microbiota and the content of butyric acid in short-chain fatty acid (SCFAs) and restoring regulatory T (Treg)/T helper type 17 (Th17) balance from the perspective of the correlation between gut microbiota and immune function.The UC rat model of spleen-kidney yang deficiency pattern was established by the method of combining disease and syndrome (intragastric administration of senna leaf, subcutaneous injection of hydrocortisone, and enema with 2,4-dinitrobenzenesulfonic acid (DNBS)/ethanol solution). After successful modeling, rats were randomly divided into six groups: the blank group, model group, low-, middle-, and high-dose Sishen Wan groups, and mesalazine group. Samples were taken after continuous administration for 3 weeks. The general conditions and body weight of the rats were observed and recorded, and the disease activity index (DAI) score was calculated. Colonic mucosal injury was observed, and a colonic mucosal damage index (CMDI) score was calculated. Histopathological changes in colon tissues were determined by hematoxylin and eosin (H&E) staining, and the histopathological score (HS) was calculated. The serum levels of transforming growth factor-β1 (TGF-β1), interleukin (IL)-6, IL-10, and IL-17 were determined by enzyme-linked immunosorbent assay (ELISA) assays. The expression of TGF-β1, signal transducer and activator of transcription 3 (STAT3), and peroxisome proliferator-activated receptor γ (PPARγ) was determined by Western blot analysis. The proportion of Th17 and Treg cells in colon tissue was determined by flow cytometry. The relative abundance of gut microbiota was determined by 16S rDNA sequencing, and the concentration of butyric acid of SCFAs was determined by gas chromatography-mass spectrometry (GC-MS).Administration of SSW significantly improved the pathological changes of colon tissue in UC rats and could attenuate the DAI and CMDI scores, and the HS. SSW significantly decreased the serum levels of IL-6 and IL-17 and increased the serum levels of TGF-β1 and IL-10. In addition, SSW increased the expression of TGF-β1 and PPARγ and decreased the expression of STAT3 in colon tissue in a dose-dependent manner. Furthermore, SSW significantly decreased the proportion of Th17 cells and increased the proportion of Treg cells in colon tissue. Additionally, SSW altered the gut microbiota, including an increase in the relative abundance of Firmicutes and a decrease in Bacteroidota at the phylum level and an increase in the relative abundance of Lactobacillus at the genus level. Moreover, SSW significantly increased the concentration of butyric acid.Combined, these data suggested that SSW increased the relative abundance of firmicutes and the level of butyric acid and restored the balance of Treg/Th17 immune axis and gut homeostasis, thus delaying the progress of UC." @default.
• W4313410109 created "2023-01-06" @default.
• W4313410109 creator A5000060166 @default.
• W4313410109 creator A5024875835 @default.
• W4313410109 creator A5041299632 @default.
• W4313410109 creator A5044383328 @default.
• W4313410109 creator A5062039431 @default.
• W4313410109 creator A5068252474 @default.
• W4313410109 date "2022-12-30" @default.
• W4313410109 modified "2023-10-09" @default.
• W4313410109 title "Sishen Wan Treats Ulcerative Colitis in Rats by Regulating Gut Microbiota and Restoring the Treg/Th17 Balance" @default.
• W4313410109 cites W1193831576 @default.
• W4313410109 cites W19574639 @default.
• W4313410109 cites W1967319082 @default.
• W4313410109 cites W1969709780 @default.
• W4313410109 cites W2002477604 @default.
• W4313410109 cites W2014750930 @default.
• W4313410109 cites W2025394958 @default.
• W4313410109 cites W2025743336 @default.
• W4313410109 cites W2026410196 @default.
• W4313410109 cites W2029383851 @default.
• W4313410109 cites W2034706392 @default.
• W4313410109 cites W2039907390 @default.
• W4313410109 cites W2050920580 @default.
• W4313410109 cites W2063230219 @default.
• W4313410109 cites W2065422693 @default.
• W4313410109 cites W2072288157 @default.
• W4313410109 cites W2089585918 @default.
• W4313410109 cites W2123166427 @default.
• W4313410109 cites W2126538938 @default.
• W4313410109 cites W2127256710 @default.
• W4313410109 cites W2133301348 @default.
• W4313410109 cites W2138372487 @default.
• W4313410109 cites W2150439294 @default.
• W4313410109 cites W2155770880 @default.
• W4313410109 cites W2271934494 @default.
• W4313410109 cites W2334373746 @default.
• W4313410109 cites W2563505151 @default.
• W4313410109 cites W2586155766 @default.
• W4313410109 cites W2596074861 @default.
• W4313410109 cites W2603846877 @default.
• W4313410109 cites W2614285633 @default.
• W4313410109 cites W2619267291 @default.
• W4313410109 cites W2764253139 @default.
• W4313410109 cites W2773996613 @default.
• W4313410109 cites W2777011462 @default.
• W4313410109 cites W2782401411 @default.
• W4313410109 cites W2791371888 @default.
• W4313410109 cites W2793380752 @default.
• W4313410109 cites W2795252759 @default.
• W4313410109 cites W2886213241 @default.
• W4313410109 cites W2889386314 @default.
• W4313410109 cites W2889535285 @default.
• W4313410109 cites W2896468689 @default.
• W4313410109 cites W2904030626 @default.
• W4313410109 cites W2908667100 @default.
• W4313410109 cites W2910577978 @default.
• W4313410109 cites W2919372986 @default.
• W4313410109 cites W2921966151 @default.
• W4313410109 cites W2922085442 @default.
• W4313410109 cites W2923739184 @default.
• W4313410109 cites W2924866833 @default.
• W4313410109 cites W2951971718 @default.
• W4313410109 cites W2968773649 @default.
• W4313410109 cites W2976362764 @default.
• W4313410109 cites W3002725495 @default.
• W4313410109 cites W3003935489 @default.
• W4313410109 cites W3008178604 @default.
• W4313410109 cites W3012963315 @default.
• W4313410109 cites W3015217980 @default.
• W4313410109 cites W3016070640 @default.
• W4313410109 cites W3020407014 @default.
• W4313410109 cites W3028868021 @default.
• W4313410109 cites W3040685676 @default.
• W4313410109 cites W3105421847 @default.
• W4313410109 cites W3112156861 @default.
• W4313410109 cites W3134702952 @default.
• W4313410109 cites W3156918339 @default.
• W4313410109 cites W4211018795 @default.
• W4313410109 cites W4211050664 @default.
• W4313410109 cites W4211138554 @default.
• W4313410109 cites W4233621198 @default.
• W4313410109 doi "https://doi.org/10.1155/2022/1432816" @default.
• W4313410109 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36619196" @default.
• W4313410109 hasPublicationYear "2022" @default.
• W4313410109 type Work @default.
• W4313410109 citedByCount "3" @default.
• W4313410109 countsByYear W43134101092023 @default.
• W4313410109 crossrefType "journal-article" @default.
• W4313410109 hasAuthorship W4313410109A5000060166 @default.
• W4313410109 hasAuthorship W4313410109A5024875835 @default.
• W4313410109 hasAuthorship W4313410109A5041299632 @default.
• W4313410109 hasAuthorship W4313410109A5044383328 @default.
• W4313410109 hasAuthorship W4313410109A5062039431 @default.
• W4313410109 hasAuthorship W4313410109A5068252474 @default.
• W4313410109 hasBestOaLocation W43134101091 @default.
• W4313410109 hasConcept C104317684 @default.
• W4313410109 hasConcept C125473707 @default.

• next