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- W4313414325 abstract "Protein display, secretion, and export in prokaryotes are essential for utilizing microbial systems as engineered living materials, medicines, biocatalysts, and protein factories. To select for improved signal peptides for Escherichia coli protein display, we utilized error-prone polymerase chain reaction (epPCR) coupled with single-cell sorting and microplate titer to generate, select, and detect improved Ag43 signal peptides. Through just three rounds of mutagenesis and selection using green fluorescence from the 56 kDa sfGFP-beta-lactamase, we isolated clones that modestly increased surface display from 1.4- to 3-fold as detected by the microplate plate-reader and native SDS-PAGE assays. To establish that the functional protein was displayed extracellularly, we trypsinized the bacterial cells to release the surface displayed proteins for analysis. This workflow demonstrated a fast and high-throughput method leveraging epPCR and single-cell sorting to augment bacterial surface display rapidly that could be applied to other bacterial proteins." @default.
- W4313414325 created "2023-01-06" @default.
- W4313414325 creator A5038527769 @default.
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- W4313414325 date "2022-12-23" @default.
- W4313414325 modified "2023-10-18" @default.
- W4313414325 title "Engineering Ag43 Signal Peptides with Bacterial Display and Selection" @default.
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- W4313414325 doi "https://doi.org/10.3390/mps6010001" @default.
- W4313414325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36648950" @default.
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