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• W4313424506 endingPage "1054" @default.
• W4313424506 startingPage "1038" @default.
• W4313424506 abstract "Abstract Nanoparticles are used as carriers for the delivery of drugs and imaging agents. Proteins are safer than synthetic nanocarriers due to their greater biocompatibility and the absence of toxic degradation products. In this context, ferritin has the additional benefit of inherently targeting the membrane receptor transferrin 1, which is overexpressed by most cancer cells. Furthermore, this self‐assembling multimeric protein can be loaded with more than 2000 iron atoms, as well as drugs, contrast agents, and other cargos. However, recombinant ferritin currently costs ~3.5 million € g −1 , presumably because the limited number of producers cannot meet demand, making it generally unaffordable as a nanocarrier. Because plants can produce proteins at very‐large‐scale, we developed a simple, proof‐of‐concept process for the production of the human ferritin heavy chain by transient expression in Nicotiana benthamiana . We optimized the protein yields by screening different compartments and 5′‐untranslated regions in PCPs, and selected the best‐performing construct for production in differentiated plants. We then established a rapid and scalable purification protocol by combining pH and heat treatment before extraction, followed by an ultrafiltration/diafiltration size‐based separation process. The optimized process achieved ferritin levels of ~40 mg kg −1 fresh biomass although depth filtration limited product recovery to ~7%. The purity of the recombinant product was >90% at costs ~3% of the current sales price. Our method therefore allows the production of affordable ferritin heavy chain as a carrier for therapeutic and diagnostic agents, which is suitable for further stability and functionality testing in vitro and in vivo." @default.
• W4313424506 created "2023-01-06" @default.
• W4313424506 creator A5003702712 @default.
• W4313424506 creator A5021076377 @default.
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• W4313424506 creator A5080120118 @default.
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• W4313424506 creator A5082891403 @default.
• W4313424506 date "2023-01-02" @default.
• W4313424506 modified "2023-10-14" @default.
• W4313424506 title "Simple plant‐based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor‐targeted drug delivery and bioimaging in cancer therapy" @default.
• W4313424506 cites W107650221 @default.
• W4313424506 cites W1454469281 @default.
• W4313424506 cites W1502384597 @default.
• W4313424506 cites W1556929642 @default.
• W4313424506 cites W1567301773 @default.
• W4313424506 cites W1969018013 @default.
• W4313424506 cites W1971949417 @default.
• W4313424506 cites W2006751824 @default.
• W4313424506 cites W2010962189 @default.
• W4313424506 cites W2015121141 @default.
• W4313424506 cites W2019228162 @default.
• W4313424506 cites W2021583384 @default.
• W4313424506 cites W2025878569 @default.
• W4313424506 cites W2026582653 @default.
• W4313424506 cites W2027836768 @default.
• W4313424506 cites W2031539675 @default.
• W4313424506 cites W2036854621 @default.
• W4313424506 cites W2037936835 @default.
• W4313424506 cites W2040043746 @default.
• W4313424506 cites W2051669323 @default.
• W4313424506 cites W2060069279 @default.
• W4313424506 cites W2063416992 @default.
• W4313424506 cites W2065455677 @default.
• W4313424506 cites W2075302528 @default.
• W4313424506 cites W2078660310 @default.
• W4313424506 cites W2081845177 @default.
• W4313424506 cites W2083224754 @default.
• W4313424506 cites W2083984579 @default.
• W4313424506 cites W2086567353 @default.
• W4313424506 cites W2087782895 @default.
• W4313424506 cites W2090205132 @default.
• W4313424506 cites W2090578893 @default.
• W4313424506 cites W2095412415 @default.
• W4313424506 cites W2104479785 @default.
• W4313424506 cites W2104756056 @default.
• W4313424506 cites W2107410272 @default.
• W4313424506 cites W2117339718 @default.
• W4313424506 cites W2128199524 @default.
• W4313424506 cites W2137122846 @default.
• W4313424506 cites W2143288895 @default.
• W4313424506 cites W2153839710 @default.
• W4313424506 cites W2154241726 @default.
• W4313424506 cites W2178800801 @default.
• W4313424506 cites W2184577383 @default.
• W4313424506 cites W2232913328 @default.
• W4313424506 cites W2241030903 @default.
• W4313424506 cites W2253053879 @default.
• W4313424506 cites W2273379493 @default.
• W4313424506 cites W2305771377 @default.
• W4313424506 cites W2344831380 @default.
• W4313424506 cites W2347184327 @default.
• W4313424506 cites W2487128806 @default.
• W4313424506 cites W2515501492 @default.
• W4313424506 cites W2565765559 @default.
• W4313424506 cites W2591416052 @default.
• W4313424506 cites W2602116138 @default.
• W4313424506 cites W2753211689 @default.
• W4313424506 cites W2756120141 @default.
• W4313424506 cites W2762341360 @default.
• W4313424506 cites W2774353159 @default.
• W4313424506 cites W2784650400 @default.
• W4313424506 cites W2786352100 @default.
• W4313424506 cites W2791330743 @default.
• W4313424506 cites W2795569119 @default.
• W4313424506 cites W2797795421 @default.
• W4313424506 cites W2807694747 @default.
• W4313424506 cites W2808290403 @default.
• W4313424506 cites W2884250078 @default.
• W4313424506 cites W2887732440 @default.
• W4313424506 cites W2895513119 @default.
• W4313424506 cites W2901925033 @default.
• W4313424506 cites W2905226814 @default.
• W4313424506 cites W2908598815 @default.
• W4313424506 cites W2914327083 @default.
• W4313424506 cites W2968787878 @default.
• W4313424506 cites W2969915597 @default.
• W4313424506 cites W2971579444 @default.
• W4313424506 cites W2972088344 @default.
• W4313424506 cites W2972461983 @default.
• W4313424506 cites W2989684857 @default.
• W4313424506 cites W3012174137 @default.
• W4313424506 cites W3021535199 @default.
• W4313424506 cites W3021746440 @default.
• W4313424506 cites W3037601887 @default.
• W4313424506 cites W3040057801 @default.
• W4313424506 cites W3043053743 @default.

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