Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313426677> ?p ?o ?g. }
- W4313426677 abstract "Abstract The stringent response, which leads to persistence of nutrient-starved mycobacteria, is induced by activation of the RelA/SpoT homologue (Rsh) upon entry of a deacylated-tRNA in a translating ribosome. However, the mechanism by which Rsh identifies such ribosomes in vivo remains unclear. Here, we show that conditions inducing ribosome hibernation result in loss of intracellular Rsh in a Clp protease-dependent manner. This loss is also observed in non-starved cells using mutations in Rsh that block its interaction with the ribosome, indicating that Rsh association with the ribosome is important for Rsh stability. The cryo-EM structure of the Rsh-bound 70S ribosome in a translation initiation complex reveals unknown interactions between the ACT domain of Rsh and components of the ribosomal L7/L12-stalk base, suggesting that the aminoacylation status of A-site tRNA is surveyed during the first cycle of elongation. Altogether, we propose a surveillance model of Rsh activation that originates from its constitutive interaction with the ribosomes entering the translation cycle. Significance Bacteria persist under nutrient starvation by activating RelA/SpoT homologue (Rsh), which synthesizes a growth regulating alarmone, ppGpp. Rsh is activated specifically upon recognizing a translation elongation complex with deacylated tRNA at the A-site. It is however unclear how Rsh identifies such a complex in vivo. We show here that conditions inducing ribosome hibernation in mycobacteria cause loss of intracellular Rsh, implying that association with translating ribosomes is necessary for intracellular stability of Rsh. Using structural analysis of Rsh-bound 70S translation initiation complex, we propose here that mycobacterial Rsh identifies a Rsh-activating ribosomal complex by constitutively surveying the ribosome entering the translation cycle at the early elongation stage." @default.
- W4313426677 created "2023-01-06" @default.
- W4313426677 creator A5024476517 @default.
- W4313426677 creator A5027702115 @default.
- W4313426677 creator A5028082814 @default.
- W4313426677 creator A5051498825 @default.
- W4313426677 creator A5052738583 @default.
- W4313426677 creator A5053602558 @default.
- W4313426677 creator A5058010245 @default.
- W4313426677 creator A5065138202 @default.
- W4313426677 creator A5065930076 @default.
- W4313426677 creator A5069939992 @default.
- W4313426677 creator A5076801513 @default.
- W4313426677 creator A5079849215 @default.
- W4313426677 creator A5089964310 @default.
- W4313426677 date "2022-12-30" @default.
- W4313426677 modified "2023-09-26" @default.
- W4313426677 title "Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation" @default.
- W4313426677 cites W176521568 @default.
- W4313426677 cites W1964979713 @default.
- W4313426677 cites W1971507610 @default.
- W4313426677 cites W1976558921 @default.
- W4313426677 cites W1986842298 @default.
- W4313426677 cites W2008186432 @default.
- W4313426677 cites W2017016334 @default.
- W4313426677 cites W2028812045 @default.
- W4313426677 cites W2034876354 @default.
- W4313426677 cites W2038871597 @default.
- W4313426677 cites W2039859631 @default.
- W4313426677 cites W2047688019 @default.
- W4313426677 cites W2058875960 @default.
- W4313426677 cites W2064578221 @default.
- W4313426677 cites W2078248420 @default.
- W4313426677 cites W2081501734 @default.
- W4313426677 cites W2081745138 @default.
- W4313426677 cites W2087905945 @default.
- W4313426677 cites W2091915244 @default.
- W4313426677 cites W2096953812 @default.
- W4313426677 cites W2097225375 @default.
- W4313426677 cites W2114972602 @default.
- W4313426677 cites W2116738290 @default.
- W4313426677 cites W2124026197 @default.
- W4313426677 cites W2125301321 @default.
- W4313426677 cites W2125391832 @default.
- W4313426677 cites W2129096064 @default.
- W4313426677 cites W2130941758 @default.
- W4313426677 cites W2132629607 @default.
- W4313426677 cites W2138611595 @default.
- W4313426677 cites W2161386390 @default.
- W4313426677 cites W2162947376 @default.
- W4313426677 cites W2164856850 @default.
- W4313426677 cites W2170273377 @default.
- W4313426677 cites W2180229411 @default.
- W4313426677 cites W2296905753 @default.
- W4313426677 cites W2345996245 @default.
- W4313426677 cites W2401547253 @default.
- W4313426677 cites W2482575482 @default.
- W4313426677 cites W2489579440 @default.
- W4313426677 cites W2593511418 @default.
- W4313426677 cites W2793041982 @default.
- W4313426677 cites W2795573442 @default.
- W4313426677 cites W2808349736 @default.
- W4313426677 cites W2816112740 @default.
- W4313426677 cites W2883603281 @default.
- W4313426677 cites W2915420499 @default.
- W4313426677 cites W2925280433 @default.
- W4313426677 cites W2949607783 @default.
- W4313426677 cites W2953320394 @default.
- W4313426677 cites W2967712258 @default.
- W4313426677 cites W2970400983 @default.
- W4313426677 cites W2972328832 @default.
- W4313426677 cites W3007658656 @default.
- W4313426677 cites W3012560802 @default.
- W4313426677 cites W3021959262 @default.
- W4313426677 cites W3037813088 @default.
- W4313426677 cites W3045835921 @default.
- W4313426677 cites W3047350338 @default.
- W4313426677 cites W3083406432 @default.
- W4313426677 cites W3085546100 @default.
- W4313426677 cites W3091866191 @default.
- W4313426677 cites W3094868867 @default.
- W4313426677 cites W3118149301 @default.
- W4313426677 cites W3133987108 @default.
- W4313426677 cites W3177828909 @default.
- W4313426677 cites W3181875588 @default.
- W4313426677 doi "https://doi.org/10.1101/2022.12.29.522164" @default.
- W4313426677 hasPublicationYear "2022" @default.
- W4313426677 type Work @default.
- W4313426677 citedByCount "0" @default.
- W4313426677 crossrefType "posted-content" @default.
- W4313426677 hasAuthorship W4313426677A5024476517 @default.
- W4313426677 hasAuthorship W4313426677A5027702115 @default.
- W4313426677 hasAuthorship W4313426677A5028082814 @default.
- W4313426677 hasAuthorship W4313426677A5051498825 @default.
- W4313426677 hasAuthorship W4313426677A5052738583 @default.
- W4313426677 hasAuthorship W4313426677A5053602558 @default.
- W4313426677 hasAuthorship W4313426677A5058010245 @default.
- W4313426677 hasAuthorship W4313426677A5065138202 @default.
- W4313426677 hasAuthorship W4313426677A5065930076 @default.
- W4313426677 hasAuthorship W4313426677A5069939992 @default.