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- W4313428149 abstract "Abstract Naive T cell activation in secondary lymphoid organs such as lymph nodes (LNs) occurs upon recognition of cognate antigens presented by antigen presenting cells (APCs). T cell activation requires cytoskeleton rearrangement and sustained interactions with APCs. Ena/VASP proteins are a family of cytoskeletal effector proteins responsible for actin polymerization and are frequently found at the leading edge of motile cells. Ena/VASP proteins have been implicated in motility and adhesion in various cell types, but their role in primary T cell activation has not been explored. Our goal was to determine the contribution of Ena/VASP proteins to T cell activation and expansion in vivo. Our results showed that naïve T cells from Ena/VASP-deficient mice have a significant reduction in antigen-specific T cell accumulation following Listeria monocytogenes infection. The kinetics of antigen-specific T cell impairment were further confirmed in Ena/VASP-deficient T cells stimulated via dendritic cell immunization. To investigate the cause of this T cell expansion defect, we analyzed T cell-APC interactions in vivo by 2-photon microscopy and observed fewer Ena/VASP-deficient naïve T cells interacting with APCs in LNs during priming. We also found that Ena/VASP-deficient T cells formed conjugates with significantly less actin polymerization at the T cell-APC synapse, and that these conjugates were less stable than their WT counterparts. Thus, we conclude that Ena/VASP proteins contribute to T cell actin remodeling downstream of T-APC interactions required for the initiation of stable T cell conjugates during APC scanning and for efficient activation and expansion of T cells in vivo. Supported by NIH (R01AI125553; T32AI007405)" @default.
- W4313428149 created "2023-01-06" @default.
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- W4313428149 date "2022-05-01" @default.
- W4313428149 modified "2023-09-27" @default.
- W4313428149 title "Ena/VASP protein-mediated actin polymerization contributes to naive CD8+ T cell activation and expansion by promoting T cell-APC interactions <i>in vivo</i>" @default.
- W4313428149 doi "https://doi.org/10.4049/jimmunol.208.supp.110.17" @default.
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