Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313432357> ?p ?o ?g. }
- W4313432357 abstract "Abstract Aberrant protein aggregation leads to various human diseases, but little is known about the physical chemical properties of these aggregated proteins in cells. Herein, we developed a boron‐dipyrromethene (BODIPY)‐based HaloTag probe, whose conjugation to HaloTag‐fused proteins allows us to study protein aggregates using both fluorescence intensity and lifetime. Modulation of BODIPY fluorophore reveals key structural features to attain the dual function. The optimized probe exhibits increased fluorescence intensity and elongated fluorescence lifetime in protein aggregates. Fluorescence lifetime imaging using this probe indicates that protein aggregates afford different viscosity in the forms of soluble oligomers and insoluble aggregates in live cells. The strategy presented in this work can be extended to enable a wide class of HaloTag probes that can be used to study a variety of physical properties of protein aggregates, thus helping unravel the pathogenic mechanism and develop therapeutic strategy." @default.
- W4313432357 created "2023-01-06" @default.
- W4313432357 creator A5006651636 @default.
- W4313432357 creator A5018073672 @default.
- W4313432357 creator A5026954484 @default.
- W4313432357 creator A5027050175 @default.
- W4313432357 creator A5047588075 @default.
- W4313432357 creator A5064873511 @default.
- W4313432357 creator A5066440529 @default.
- W4313432357 creator A5068061602 @default.
- W4313432357 date "2022-12-19" @default.
- W4313432357 modified "2023-09-30" @default.
- W4313432357 title "A dual‐functional BODIPY‐based molecular rotor probe reveals different viscosity of protein aggregates in live cells" @default.
- W4313432357 cites W1574404737 @default.
- W4313432357 cites W1967025628 @default.
- W4313432357 cites W1980825524 @default.
- W4313432357 cites W2001974127 @default.
- W4313432357 cites W2002559713 @default.
- W4313432357 cites W2006483299 @default.
- W4313432357 cites W2017848780 @default.
- W4313432357 cites W2027828974 @default.
- W4313432357 cites W2030936891 @default.
- W4313432357 cites W2034548105 @default.
- W4313432357 cites W2038025508 @default.
- W4313432357 cites W2050479047 @default.
- W4313432357 cites W2056192203 @default.
- W4313432357 cites W2057468776 @default.
- W4313432357 cites W2061278371 @default.
- W4313432357 cites W2076159058 @default.
- W4313432357 cites W2078942070 @default.
- W4313432357 cites W2080225612 @default.
- W4313432357 cites W2093362341 @default.
- W4313432357 cites W2096386031 @default.
- W4313432357 cites W2110399112 @default.
- W4313432357 cites W2111282783 @default.
- W4313432357 cites W2112272965 @default.
- W4313432357 cites W2130741221 @default.
- W4313432357 cites W2134178604 @default.
- W4313432357 cites W2159182565 @default.
- W4313432357 cites W2166155593 @default.
- W4313432357 cites W2167113152 @default.
- W4313432357 cites W2178439483 @default.
- W4313432357 cites W2315080745 @default.
- W4313432357 cites W2317210131 @default.
- W4313432357 cites W2332764971 @default.
- W4313432357 cites W2337585522 @default.
- W4313432357 cites W2516806610 @default.
- W4313432357 cites W2522989562 @default.
- W4313432357 cites W2567350401 @default.
- W4313432357 cites W2582420645 @default.
- W4313432357 cites W2617232863 @default.
- W4313432357 cites W2744329338 @default.
- W4313432357 cites W2752787262 @default.
- W4313432357 cites W2758039731 @default.
- W4313432357 cites W2772638709 @default.
- W4313432357 cites W2779360719 @default.
- W4313432357 cites W2792514006 @default.
- W4313432357 cites W2794833744 @default.
- W4313432357 cites W2797019411 @default.
- W4313432357 cites W2807448578 @default.
- W4313432357 cites W2908035833 @default.
- W4313432357 cites W2914378053 @default.
- W4313432357 cites W2943066132 @default.
- W4313432357 cites W2946047987 @default.
- W4313432357 cites W3086592412 @default.
- W4313432357 cites W3091138450 @default.
- W4313432357 doi "https://doi.org/10.1002/agt2.301" @default.
- W4313432357 hasPublicationYear "2022" @default.
- W4313432357 type Work @default.
- W4313432357 citedByCount "8" @default.
- W4313432357 countsByYear W43134323572023 @default.
- W4313432357 crossrefType "journal-article" @default.
- W4313432357 hasAuthorship W4313432357A5006651636 @default.
- W4313432357 hasAuthorship W4313432357A5018073672 @default.
- W4313432357 hasAuthorship W4313432357A5026954484 @default.
- W4313432357 hasAuthorship W4313432357A5027050175 @default.
- W4313432357 hasAuthorship W4313432357A5047588075 @default.
- W4313432357 hasAuthorship W4313432357A5064873511 @default.
- W4313432357 hasAuthorship W4313432357A5066440529 @default.
- W4313432357 hasAuthorship W4313432357A5068061602 @default.
- W4313432357 hasBestOaLocation W43134323571 @default.
- W4313432357 hasConcept C121332964 @default.
- W4313432357 hasConcept C12554922 @default.
- W4313432357 hasConcept C127172972 @default.
- W4313432357 hasConcept C136238340 @default.
- W4313432357 hasConcept C159985019 @default.
- W4313432357 hasConcept C171250308 @default.
- W4313432357 hasConcept C185592680 @default.
- W4313432357 hasConcept C192562407 @default.
- W4313432357 hasConcept C2778617687 @default.
- W4313432357 hasConcept C2781374752 @default.
- W4313432357 hasConcept C55493867 @default.
- W4313432357 hasConcept C62520636 @default.
- W4313432357 hasConcept C86803240 @default.
- W4313432357 hasConcept C91881484 @default.
- W4313432357 hasConceptScore W4313432357C121332964 @default.
- W4313432357 hasConceptScore W4313432357C12554922 @default.
- W4313432357 hasConceptScore W4313432357C127172972 @default.
- W4313432357 hasConceptScore W4313432357C136238340 @default.
- W4313432357 hasConceptScore W4313432357C159985019 @default.