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• W4313442557 endingPage "133245" @default.
• W4313442557 startingPage "133245" @default.
• W4313442557 abstract "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19). The high human-to-human transmission and rapid evolution of SARS-CoV-2 have resulted in a worldwide pandemic. To contain SARS-CoV-2, it is essential to efficiently control the transmission of the virus through the early diagnosis of infected individuals, including asymptomatic people. Therefore, a rapid and accurate assay is vital for the early diagnosis of SARS-CoV-2 in suspected individuals. In this study, we developed a colorimetric lateral flow immunoassay (LFIA) in which a CBP31-BC linker was used to immobilize antibodies on a cellulose membrane in an oriented manner. The developed LFIA enabled sensitive detection of cultured SARS-CoV-2 in 15 min with a detection limit of 5 × 104 copies/mL. The clinical performance of the LFIA for detecting SARS-CoV-2 was evaluated using 19 clinical samples validated by reverse transcription-polymerase chain reaction (RTPCR). The LFIA detected all the positive and negative samples accurately, corresponding to 100% accuracy. Importantly, patient samples with low viral loads were accurately identified. Thus, the proposed method can provide a useful platform for rapid and accurate point-of-care testing of SARS-CoV-2 in infected individuals to efficiently control the COVID-19 pandemic." @default.
• W4313442557 created "2023-01-06" @default.
• W4313442557 creator A5006781936 @default.
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• W4313442557 date "2023-03-01" @default.
• W4313442557 modified "2023-10-11" @default.
• W4313442557 title "A colorimetric lateral flow immunoassay based on oriented antibody immobilization for sensitive detection of SARS-CoV-2" @default.
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• W4313442557 doi "https://doi.org/10.1016/j.snb.2022.133245" @default.
• W4313442557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36589904" @default.
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