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• W4313442684 abstract "To achieve an efficient and cost-effective conversion from waste cooking oil (WCO) to biodiesel, a facile one-pot co-immobilization of Aspergillus oryzae lipase (AOL) and Rhizomucor miehei lipase (RML) was developed by rapid encapsulation of magnetic chitosan microcapsules. The encapsulation process was accomplished by self-assembly of negatively charged magnetic nanoparticles (citrate modified Fe3O4) on ionic cross-linked chitosan-citrate aggregates containing lipases. Integrating Tween 80 bio-imprinting of lipases, the co-immobilized [email protected] (co-im Tween [email protected]) achieved a significant increase in activity and reached 98.5 % biodiesel yield under optimal conditions, which was 1.3 and 1.6 times higher than that of single immobilized AOL and RML, respectively. Notably, owing to the beneficial synergistic impact of methanol-tolerable AOL and thermal-stable RML, co-im Tween [email protected] displayed superior activity over single lipase counterparts under excess methanol and variable temperatures. The co-im Tween [email protected] could maintained 96 % activity after 3 cycles of biodiesel production; however, showed an obvious inactivation after continued reuse. Moreover, methanol-induced denaturation of lipases and glycerol-caused agglomeration of the magnetic nanoparticles during continued transesterification were ascertained by fourier transform infrared analysis and scanning electron microscopy. This study provides new pathways for constructing multi-lipase co-immobilzation as well as an economical and effective biocatalyst for biodiesel production from WCO." @default.
• W4313442684 created "2023-01-06" @default.
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• W4313442684 date "2023-02-01" @default.
• W4313442684 modified "2023-10-01" @default.
• W4313442684 title "Efficient biodiesel production from waste cooking oil by fast co-immobilization of lipases from Aspergillus oryzae and Rhizomucor miehei in magnetic chitosan microcapsules" @default.
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• W4313442684 doi "https://doi.org/10.1016/j.procbio.2022.12.025" @default.
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