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- W4313446070 abstract "Numerous models have been developed for the analysis of fluorescence recovery after photobleaching (FRAP), by which intracellular diffusion and turnover rate are quantitatively evaluated. FRAP analyses typically focus on such events that occur within several minutes, but to precisely evaluate a slow turnover rate of particularly actin stress fibers, achieving long-term FRAP observations of more than 10 min is necessary. In such long-term observations, the effect of intracellular advection is no longer ignored, which motivated us to develop a novel method to decouple the multiple factors associated with the long FRAP response. This method allows us to distinguish the origin of mechanobiological responses of stress fibers that come from either the level of individual actin filaments or that of actin monomers." @default.
- W4313446070 created "2023-01-06" @default.
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- W4313446070 date "2023-01-01" @default.
- W4313446070 modified "2023-10-18" @default.
- W4313446070 title "Long-Term Fluorescence Recovery After Photobleaching (FRAP)" @default.
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- W4313446070 doi "https://doi.org/10.1007/978-1-0716-2851-5_21" @default.
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