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- W4313446186 abstract "GM3 synthase (GM3S) is a sialyltransferase that transfers sialic acid from CMP-sialic acid to lactosylceramide. This reaction results in formation of ganglioside GM3 and is essential for biosynthesis of its downstream derivatives, which include a- and b-series gangliosides. Here, we describe a method for GM3S enzymatic assay using fluorescence-labeled alkyl lactoside as acceptor substrate, followed by HPLC for separation of enzymatic product. The method allows quantitative assay of GM3S sialyltransferase activity in cultured cells and mouse brain tissues." @default.
- W4313446186 created "2023-01-06" @default.
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- W4313446186 date "2023-01-01" @default.
- W4313446186 modified "2023-09-25" @default.
- W4313446186 title "Sialyltransferase Activity Assay for Ganglioside GM3 Synthase" @default.
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- W4313446186 doi "https://doi.org/10.1007/978-1-0716-2910-9_9" @default.
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