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- W4313447659 abstract "Understanding the spatiotemporal pattern of genes involved in a specific biological process is critical for uncovering their organization, regulation, and functions. Thus, the visualization of gene expression in various tissues under distinct physiological conditions has become routine work in biology. Limited by the availability of high-quality antibodies, it is still very challenging to examine the expression of many genes that have not been well characterized. Chromogenic RNA in situ hybridization is a powerful and relatively cheap tool to characterize target RNA abundance and localization in cells and tissues. It allows rapid visualization and validation of candidate genes identified by next-generation sequencing-related technologies. Here, we present step by step a reliable and detailed chromogenic RNA in situ hybridization protocol for the emerging model African turquoise killifish Nothobranchius furzeri. Our goal is to provide an optimized and rapid procedure for investigating genes involved in African killifish development, regeneration, and aging. This protocol can also be applied to other fish species." @default.
- W4313447659 created "2023-01-06" @default.
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- W4313447659 date "2023-01-01" @default.
- W4313447659 modified "2023-09-28" @default.
- W4313447659 title "Rapid Visualization of Gene Expression Using Chromogenic RNA In Situ Hybridization in African Killifish" @default.
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- W4313447659 doi "https://doi.org/10.1007/978-1-0716-2875-1_9" @default.
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