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- W4313448542 abstract "Abstract The changing climate leads to frequent low temperature events, which results in lower crop production worldwide. Plant growth and development are regulated by WRKYs, as well as a variety of defense responses, including salt, drought, and extreme temperature stresses. Here, a transcription factor AeWRKY31 was obtained from Abelmoschus esculentus and ectopic expressed in Arabidopsis thaliana . Molecular localization and self-transactivation assays revealed AeWRKY31 was a nuclear-localized protein with self-transactivation property. Subsequently, AeWRKY31 expression activated the expression of AtABA1 , AtABA2, and AtNCED6 , and promoted endogenous ABA accumulation, which induced stomata closure of transgenic Arabidopsis. Histochemical staining and ROS content determination demonstrated that AeWRKY31 expression inhibited the accumulation of H 2 O 2 and O 2- and decrease electrolyte leakage in transgenic lines. Meanwhile, compared with that of WT, relative expression levels of cold response-related genes were up-regulated in transgenic lines. Finally, AeWRKY31 transgenic Arabidopsis plants exhibited enhanced cold tolerance both in vivo and in vitro experiments. Our findings will benefit molecular breeding and anti-cold mechanism investigation of okra." @default.
- W4313448542 created "2023-01-06" @default.
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- W4313448542 creator A5051932127 @default.
- W4313448542 creator A5053818798 @default.
- W4313448542 date "2022-12-20" @default.
- W4313448542 modified "2023-10-10" @default.
- W4313448542 title "Okra transcription factor AeWRKY31 enhanced cold resistance of transgenic Arabidopsis through promoting ABA biosynthesis and inhibiting ROS production" @default.
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- W4313448542 doi "https://doi.org/10.21203/rs.3.rs-2370753/v1" @default.
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