Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313448552> ?p ?o ?g. }
- W4313448552 abstract "Abstract The kidney plays a critical role in excreting ammonia during metabolic acidosis and liver failure. The mechanisms behind this process have been poorly explored. The present study combines results of in vivo experiments of increased total ammoniagenesis with systems biology modeling, in which eight rats were fed an amino acid-rich diet (HD group) and eight a normal chow diet (AL group). We developed a method based on elementary mode analysis to study changes in amino acid flux occurring across the kidney in increased ammoniagenesis. Elementary modes represent minimal feasible metabolic paths in steady state. The model was used to predict amino acid fluxes in healthy and pre-hyperammonemic conditions, which were compared to experimental fluxes in rats. First, we found that total renal ammoniagenesis increased from 264 ± 68 to 612 ± 87 nmol (100 g body weight) −1 min −1 in the HD group ( P = 0.021) and a concomitated upregulation of NKCC2 ammonia and other transporters in the kidney. In the kidney metabolic model, the best predictions were obtained with ammonia transport as an objective. Other objectives resulting in a fair correlation with the measured fluxes (correlation coefficient >0.5) were growth, protein uptake, urea excretion, and lysine and phenylalanine transport. These predictions were improved when specific gene expression data were considered in HD conditions, suggesting a role for the mitochondrial glycine pathway. Further studies are needed to determine if regulation through the mitochondrial glycine pathway and ammonia transporters can be modulated and how to use the kidney as a therapeutic target in hyperammonemia." @default.
- W4313448552 created "2023-01-06" @default.
- W4313448552 creator A5005055595 @default.
- W4313448552 creator A5046134592 @default.
- W4313448552 creator A5047324691 @default.
- W4313448552 creator A5051029037 @default.
- W4313448552 creator A5062633175 @default.
- W4313448552 creator A5062704912 @default.
- W4313448552 creator A5065264795 @default.
- W4313448552 creator A5076113252 @default.
- W4313448552 creator A5080608184 @default.
- W4313448552 creator A5087084918 @default.
- W4313448552 creator A5088611316 @default.
- W4313448552 date "2022-12-20" @default.
- W4313448552 modified "2023-10-18" @default.
- W4313448552 title "Fluxomics reveals cellular and molecular basis of increased renal ammoniagenesis" @default.
- W4313448552 cites W1563019103 @default.
- W4313448552 cites W1594663117 @default.
- W4313448552 cites W1606730015 @default.
- W4313448552 cites W174810609 @default.
- W4313448552 cites W1801619426 @default.
- W4313448552 cites W1969852704 @default.
- W4313448552 cites W1978232207 @default.
- W4313448552 cites W1985876751 @default.
- W4313448552 cites W1986025103 @default.
- W4313448552 cites W1993238514 @default.
- W4313448552 cites W1995880424 @default.
- W4313448552 cites W2007462231 @default.
- W4313448552 cites W2012034410 @default.
- W4313448552 cites W2019974881 @default.
- W4313448552 cites W2022664194 @default.
- W4313448552 cites W2035918887 @default.
- W4313448552 cites W2039183451 @default.
- W4313448552 cites W2039615827 @default.
- W4313448552 cites W2041167675 @default.
- W4313448552 cites W2042118510 @default.
- W4313448552 cites W2045921664 @default.
- W4313448552 cites W2045949302 @default.
- W4313448552 cites W2049822731 @default.
- W4313448552 cites W2080957738 @default.
- W4313448552 cites W2083818654 @default.
- W4313448552 cites W2094399313 @default.
- W4313448552 cites W2099163901 @default.
- W4313448552 cites W2108195113 @default.
- W4313448552 cites W2110355460 @default.
- W4313448552 cites W2113788831 @default.
- W4313448552 cites W2122678100 @default.
- W4313448552 cites W2128800957 @default.
- W4313448552 cites W2132218677 @default.
- W4313448552 cites W2135791865 @default.
- W4313448552 cites W2137242964 @default.
- W4313448552 cites W2142682640 @default.
- W4313448552 cites W2144589197 @default.
- W4313448552 cites W2155099762 @default.
- W4313448552 cites W2158069895 @default.
- W4313448552 cites W2159044433 @default.
- W4313448552 cites W2170005341 @default.
- W4313448552 cites W2170974939 @default.
- W4313448552 cites W2179438025 @default.
- W4313448552 cites W2211215064 @default.
- W4313448552 cites W2230096191 @default.
- W4313448552 cites W2267153832 @default.
- W4313448552 cites W2344119092 @default.
- W4313448552 cites W2345803218 @default.
- W4313448552 cites W2767891136 @default.
- W4313448552 cites W2795811709 @default.
- W4313448552 cites W2928665623 @default.
- W4313448552 doi "https://doi.org/10.1038/s41540-022-00257-2" @default.
- W4313448552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36539425" @default.
- W4313448552 hasPublicationYear "2022" @default.
- W4313448552 type Work @default.
- W4313448552 citedByCount "0" @default.
- W4313448552 crossrefType "journal-article" @default.
- W4313448552 hasAuthorship W4313448552A5005055595 @default.
- W4313448552 hasAuthorship W4313448552A5046134592 @default.
- W4313448552 hasAuthorship W4313448552A5047324691 @default.
- W4313448552 hasAuthorship W4313448552A5051029037 @default.
- W4313448552 hasAuthorship W4313448552A5062633175 @default.
- W4313448552 hasAuthorship W4313448552A5062704912 @default.
- W4313448552 hasAuthorship W4313448552A5065264795 @default.
- W4313448552 hasAuthorship W4313448552A5076113252 @default.
- W4313448552 hasAuthorship W4313448552A5080608184 @default.
- W4313448552 hasAuthorship W4313448552A5087084918 @default.
- W4313448552 hasAuthorship W4313448552A5088611316 @default.
- W4313448552 hasBestOaLocation W43134485521 @default.
- W4313448552 hasConcept C10146269 @default.
- W4313448552 hasConcept C126322002 @default.
- W4313448552 hasConcept C134018914 @default.
- W4313448552 hasConcept C185592680 @default.
- W4313448552 hasConcept C2777431362 @default.
- W4313448552 hasConcept C2777468819 @default.
- W4313448552 hasConcept C2777756961 @default.
- W4313448552 hasConcept C2779246250 @default.
- W4313448552 hasConcept C2779349466 @default.
- W4313448552 hasConcept C2780091579 @default.
- W4313448552 hasConcept C2780365088 @default.
- W4313448552 hasConcept C515207424 @default.
- W4313448552 hasConcept C55493867 @default.
- W4313448552 hasConcept C71924100 @default.
- W4313448552 hasConcept C77061444 @default.