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- W4313452605 abstract "We present a new label-free three-dimensional (3D) microscopy technique, termed transport of intensity diffraction tomography with non-interferometric synthetic aperture (TIDT-NSA). Without resorting to interferometric detection, TIDT-NSA retrieves the 3D refractive index (RI) distribution of biological specimens from 3D intensity-only measurements at various illumination angles, allowing incoherent-diffraction-limited quantitative 3D phase-contrast imaging. The unique combination of z-scanning the sample with illumination angle diversity in TIDT-NSA provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution tomography of complex multi-layer biological samples. Based on an off-the-shelf bright-field microscope with a programmable lightemitting-diode (LED) illumination source, we demonstrate the achievable imaging resolution of TIDT-NSA at 206 nm laterally and 0.52 um axially with a high-NA oil immersion objective and validate the 3D R" @default.
- W4313452605 created "2023-01-06" @default.
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- W4313452605 date "2023-01-04" @default.
- W4313452605 modified "2023-09-30" @default.
- W4313452605 title "Transport of intensity diffraction tomography for three-dimensional label-free microscopy under arbitrary non-matched illumination condition" @default.
- W4313452605 doi "https://doi.org/10.1117/12.2643039" @default.
- W4313452605 hasPublicationYear "2023" @default.
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