FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313453609> ?p ?o ?g. }

• W4313453609 endingPage "113397" @default.
• W4313453609 startingPage "113397" @default.
• W4313453609 abstract "Adeno-associated virus (AAV) based gene therapies are gaining significant momentum as a novel therapeutic modality. However, a yet unsolved concern for using AAV as a vector is the high potential to elicit humoral and cellular responses, which are often exacerbated by pre-existing immunity due to exposure to wild type AAV. Therefore, characterization of pre-existing and treatment emergent anti-AAV antibodies is of great importance to the development of AAV based gene therapies. In this project, a sensitive and drug tolerant total antibody (TAb) assay was developed using recombinant AAV9-GFP (green fluorescent protein) as a surrogate AAV9. The assay format was affinity capture and elution (ACE) with ruthenium labeled AAV9-GFP as detection. Upon evaluation, three commercial anti-AAV9 monoclonal antibodies (clones HI17, HI35, and HL2374) were chosen and mixed at equal concentrations as positive control material. The assay sensitivity was estimated to be 11.2 ng/mL. Drug tolerance was estimated to be 5.4 × 10E10 DRP/mL AAV9-GFP at 100 ng/mL anti-AAV9 antibodies and to be at least 1 × 10E11 DRP/mL at 500 ng/mL and 250 ng/mL anti-AAV9 antibodies. The assay showed desirable specificity and precision. Using this TAb assay, significant pre-existing antibodies were detected from normal human sera." @default.
• W4313453609 created "2023-01-06" @default.
• W4313453609 creator A5010098535 @default.
• W4313453609 creator A5023435657 @default.
• W4313453609 creator A5028973369 @default.
• W4313453609 creator A5037903899 @default.
• W4313453609 creator A5051170290 @default.
• W4313453609 creator A5062556828 @default.
• W4313453609 creator A5082420242 @default.
• W4313453609 date "2023-01-01" @default.
• W4313453609 modified "2023-10-18" @default.
• W4313453609 title "A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution" @default.
• W4313453609 cites W1966214235 @default.
• W4313453609 cites W1969271703 @default.
• W4313453609 cites W1976329054 @default.
• W4313453609 cites W2007261697 @default.
• W4313453609 cites W2055995773 @default.
• W4313453609 cites W2067204822 @default.
• W4313453609 cites W2070447470 @default.
• W4313453609 cites W2071738937 @default.
• W4313453609 cites W2082025265 @default.
• W4313453609 cites W2095942414 @default.
• W4313453609 cites W2122304523 @default.
• W4313453609 cites W2130515212 @default.
• W4313453609 cites W2138117792 @default.
• W4313453609 cites W2149757665 @default.
• W4313453609 cites W2164545730 @default.
• W4313453609 cites W2170205772 @default.
• W4313453609 cites W2170549121 @default.
• W4313453609 cites W2253966500 @default.
• W4313453609 cites W2401533913 @default.
• W4313453609 cites W2464400647 @default.
• W4313453609 cites W2464528200 @default.
• W4313453609 cites W2736065260 @default.
• W4313453609 cites W2746053108 @default.
• W4313453609 cites W2765408594 @default.
• W4313453609 cites W2767505578 @default.
• W4313453609 cites W2793421058 @default.
• W4313453609 cites W2883141789 @default.
• W4313453609 cites W2890153958 @default.
• W4313453609 cites W2894600652 @default.
• W4313453609 cites W2896929454 @default.
• W4313453609 cites W2897401411 @default.
• W4313453609 cites W2913212173 @default.
• W4313453609 cites W2947983278 @default.
• W4313453609 cites W2957860328 @default.
• W4313453609 cites W2995820186 @default.
• W4313453609 cites W2998050720 @default.
• W4313453609 cites W3000219265 @default.
• W4313453609 cites W3004887743 @default.
• W4313453609 cites W3035047148 @default.
• W4313453609 cites W3120585537 @default.
• W4313453609 cites W3124760643 @default.
• W4313453609 cites W3135935053 @default.
• W4313453609 cites W3198940616 @default.
• W4313453609 cites W4213418485 @default.
• W4313453609 cites W4226258165 @default.
• W4313453609 cites W4250446838 @default.
• W4313453609 doi "https://doi.org/10.1016/j.jim.2022.113397" @default.
• W4313453609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36481208" @default.
• W4313453609 hasPublicationYear "2023" @default.
• W4313453609 type Work @default.
• W4313453609 citedByCount "2" @default.
• W4313453609 countsByYear W43134536092023 @default.
• W4313453609 crossrefType "journal-article" @default.
• W4313453609 hasAuthorship W4313453609A5010098535 @default.
• W4313453609 hasAuthorship W4313453609A5023435657 @default.
• W4313453609 hasAuthorship W4313453609A5028973369 @default.
• W4313453609 hasAuthorship W4313453609A5037903899 @default.
• W4313453609 hasAuthorship W4313453609A5051170290 @default.
• W4313453609 hasAuthorship W4313453609A5062556828 @default.
• W4313453609 hasAuthorship W4313453609A5082420242 @default.
• W4313453609 hasConcept C104317684 @default.
• W4313453609 hasConcept C153911025 @default.
• W4313453609 hasConcept C159047783 @default.
• W4313453609 hasConcept C159654299 @default.
• W4313453609 hasConcept C179247698 @default.
• W4313453609 hasConcept C181199279 @default.
• W4313453609 hasConcept C203014093 @default.
• W4313453609 hasConcept C40767141 @default.
• W4313453609 hasConcept C542903549 @default.
• W4313453609 hasConcept C55493867 @default.
• W4313453609 hasConcept C86803240 @default.
• W4313453609 hasConceptScore W4313453609C104317684 @default.
• W4313453609 hasConceptScore W4313453609C153911025 @default.
• W4313453609 hasConceptScore W4313453609C159047783 @default.
• W4313453609 hasConceptScore W4313453609C159654299 @default.
• W4313453609 hasConceptScore W4313453609C179247698 @default.
• W4313453609 hasConceptScore W4313453609C181199279 @default.
• W4313453609 hasConceptScore W4313453609C203014093 @default.
• W4313453609 hasConceptScore W4313453609C40767141 @default.
• W4313453609 hasConceptScore W4313453609C542903549 @default.
• W4313453609 hasConceptScore W4313453609C55493867 @default.
• W4313453609 hasConceptScore W4313453609C86803240 @default.
• W4313453609 hasFunder F4320307758 @default.
• W4313453609 hasLocation W43134536091 @default.
• W4313453609 hasLocation W43134536092 @default.
• W4313453609 hasOpenAccess W4313453609 @default.

• next