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• W4313456445 abstract "Abstract Several nuclear proteins undergo condensation. The question remains often whether this property is coupled to a functional aspect of the protein in the nucleus. The basic helix-loop-helix (bHLH) FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) integrates internal and external signals to control the amount of iron that is acquired in accordance with growth. The previously described C-terminal Ser271/272 allows FIT to form active complexes with subgroup Ib bHLH factors such as bHLH039. FIT has lower nuclear mobility than mutant FITmSS271AA, but this behavior has remained mechanistically and functionally obscure. Here, we show that FIT undergoes a light-inducible subnuclear partitioning into nuclear condensates that we termed FIT nuclear bodies (NBs). The characteristics of FIT NBs could be examined using a standardized FIT NB analysis procedure coupled with different types of quantitative and qualitative microscopy-based approaches. We found that FIT condensates were likely formed by liquid-liquid phase separation. FIT accumulated preferentially in FIT NBs versus nucleoplasm when engaged in protein complexes with itself and with bHLH039. FITmSS271AA, instead, localized to NBs with different dynamics. FIT colocalized with splicing and light signaling NB markers. Hence, the inducible highly dynamic FIT condensates link active transcription factor complexes with posttranscriptional regulation processes. Highlights FIT undergoes light-induced condensation and localizes to NBs, likely via LLPS Functionally relevant Ser271/272 defines an intrinsically disordered region and influences NB formation dynamics NBs are preferential sites for FIT dimerization with FIT and bHLH039, dependent on Ser271/272 FIT NBs colocalize with NB markers related to splicing and light signalling" @default.
• W4313456445 created "2023-01-06" @default.
• W4313456445 creator A5022617095 @default.
• W4313456445 creator A5032627039 @default.
• W4313456445 creator A5034789705 @default.
• W4313456445 creator A5053265638 @default.
• W4313456445 creator A5080365283 @default.
• W4313456445 creator A5088362029 @default.
• W4313456445 date "2022-12-20" @default.
• W4313456445 modified "2023-10-14" @default.
• W4313456445 title "FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) accumulates in homo- and heterodimeric complexes in dynamic and inducible nuclear condensates associated with speckle components" @default.
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• W4313456445 doi "https://doi.org/10.1101/2022.12.20.520918" @default.
• W4313456445 hasPublicationYear "2022" @default.
• W4313456445 type Work @default.
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• W4313456445 hasAuthorship W4313456445A5022617095 @default.
• W4313456445 hasAuthorship W4313456445A5032627039 @default.
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• W4313456445 hasAuthorship W4313456445A5053265638 @default.
• W4313456445 hasAuthorship W4313456445A5080365283 @default.

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