Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313456747> ?p ?o ?g. }
- W4313456747 abstract "Large-scale synthesis of GM1, an important ganglioside in mammalian cells especially those in the nervous system, is needed to explore its therapeutic potential. Biocatalytic production is a promising platform for such a purpose. We report herein the development of process engineering and glycosyltransferase improvement strategies to advance chemoenzymatic total synthesis of GM1. Firstly, a new short route was developed for chemical synthesis of lactosylsphingosine from the commercially available Garner's aldehyde. Secondly, two glycosyltransferases including Campylobacter jejuni β1-4GalNAcT (CjCgtA) and β1-3-galactosyltransferase (CjCgtB) were improved on their soluble expression in E. coli and enzyme stability by fusing with an N-terminal maltose binding protein (MBP). Thirdly, the process for enzymatic synthesis of GM1 sphingosines from lactosylsphingosine was engineered by developing a multistep one-pot multienzyme (MSOPME) strategy without isolating intermediate glycosphingosines and by adding a detergent, sodium cholate, to the later enzymatic glycosylation steps. Installation of a desired fatty acyl chain to GM1 glycosphingosines led to the formation of target GM1 gangliosides. The combination of glycosyltransferase improvement with chemical and enzymatic process engineering represents a significant advance in obtaining GM1 gangliosides containing different sialic acid forms by total chemoenzymatic synthesis in a short route and with high efficiency." @default.
- W4313456747 created "2023-01-06" @default.
- W4313456747 creator A5008171524 @default.
- W4313456747 creator A5012704417 @default.
- W4313456747 creator A5018882725 @default.
- W4313456747 creator A5027313200 @default.
- W4313456747 creator A5060090292 @default.
- W4313456747 date "2023-03-17" @default.
- W4313456747 modified "2023-10-15" @default.
- W4313456747 title "Process Engineering and Glycosyltransferase Improvement for Short Route Chemoenzymatic Total Synthesis of GM1 Gangliosides" @default.
- W4313456747 cites W131247432 @default.
- W4313456747 cites W1487028966 @default.
- W4313456747 cites W1495217326 @default.
- W4313456747 cites W1963845059 @default.
- W4313456747 cites W1981464833 @default.
- W4313456747 cites W1984187371 @default.
- W4313456747 cites W1984808884 @default.
- W4313456747 cites W1987757704 @default.
- W4313456747 cites W1991821447 @default.
- W4313456747 cites W1998487735 @default.
- W4313456747 cites W2003681003 @default.
- W4313456747 cites W2003832582 @default.
- W4313456747 cites W2013495203 @default.
- W4313456747 cites W2016613302 @default.
- W4313456747 cites W2018902553 @default.
- W4313456747 cites W2020030959 @default.
- W4313456747 cites W2028429391 @default.
- W4313456747 cites W2029628150 @default.
- W4313456747 cites W2037061238 @default.
- W4313456747 cites W2038368280 @default.
- W4313456747 cites W2039421884 @default.
- W4313456747 cites W2045902043 @default.
- W4313456747 cites W2054933711 @default.
- W4313456747 cites W2089598495 @default.
- W4313456747 cites W2093233484 @default.
- W4313456747 cites W2124864045 @default.
- W4313456747 cites W2138690460 @default.
- W4313456747 cites W2156560231 @default.
- W4313456747 cites W2174040273 @default.
- W4313456747 cites W2463514561 @default.
- W4313456747 cites W2530923045 @default.
- W4313456747 cites W2731681260 @default.
- W4313456747 cites W2731681467 @default.
- W4313456747 cites W2793149852 @default.
- W4313456747 cites W2795058338 @default.
- W4313456747 cites W2800613529 @default.
- W4313456747 cites W2802827513 @default.
- W4313456747 cites W2897424205 @default.
- W4313456747 cites W2995655607 @default.
- W4313456747 cites W2999554735 @default.
- W4313456747 cites W3003365674 @default.
- W4313456747 cites W3009486254 @default.
- W4313456747 cites W3036063688 @default.
- W4313456747 cites W3130341140 @default.
- W4313456747 cites W3165030394 @default.
- W4313456747 cites W3172060644 @default.
- W4313456747 cites W3174475254 @default.
- W4313456747 cites W3208693887 @default.
- W4313456747 cites W3213442916 @default.
- W4313456747 cites W4205220503 @default.
- W4313456747 cites W4206297637 @default.
- W4313456747 cites W4207076926 @default.
- W4313456747 cites W4210636183 @default.
- W4313456747 cites W4210805479 @default.
- W4313456747 cites W4211084424 @default.
- W4313456747 cites W4225308535 @default.
- W4313456747 cites W4319308111 @default.
- W4313456747 doi "https://doi.org/10.1002/chem.202300005" @default.
- W4313456747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36596720" @default.
- W4313456747 hasPublicationYear "2023" @default.
- W4313456747 type Work @default.
- W4313456747 citedByCount "2" @default.
- W4313456747 countsByYear W43134567472023 @default.
- W4313456747 crossrefType "journal-article" @default.
- W4313456747 hasAuthorship W4313456747A5008171524 @default.
- W4313456747 hasAuthorship W4313456747A5012704417 @default.
- W4313456747 hasAuthorship W4313456747A5018882725 @default.
- W4313456747 hasAuthorship W4313456747A5027313200 @default.
- W4313456747 hasAuthorship W4313456747A5060090292 @default.
- W4313456747 hasConcept C147816474 @default.
- W4313456747 hasConcept C161019410 @default.
- W4313456747 hasConcept C181199279 @default.
- W4313456747 hasConcept C185592680 @default.
- W4313456747 hasConcept C2777331542 @default.
- W4313456747 hasConcept C2780115458 @default.
- W4313456747 hasConcept C2780895608 @default.
- W4313456747 hasConcept C55493867 @default.
- W4313456747 hasConcept C6350086 @default.
- W4313456747 hasConceptScore W4313456747C147816474 @default.
- W4313456747 hasConceptScore W4313456747C161019410 @default.
- W4313456747 hasConceptScore W4313456747C181199279 @default.
- W4313456747 hasConceptScore W4313456747C185592680 @default.
- W4313456747 hasConceptScore W4313456747C2777331542 @default.
- W4313456747 hasConceptScore W4313456747C2780115458 @default.
- W4313456747 hasConceptScore W4313456747C2780895608 @default.
- W4313456747 hasConceptScore W4313456747C55493867 @default.
- W4313456747 hasConceptScore W4313456747C6350086 @default.
- W4313456747 hasFunder F4320337349 @default.
- W4313456747 hasFunder F4320337354 @default.
- W4313456747 hasIssue "25" @default.