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- W4313457531 abstract "Abstract Optogenetics is a powerful tool for manipulating neuronal activity by light illumination with high temporal and spatial resolution. Anion-channelrhodopsins (ACRs) are light-gated anion channels that allow researchers to efficiently inhibit neuronal activity. A blue light-sensitive ACR2 has recently been used in several in vivo studies; however, the reporter mouse strain expressing ACR2 has not yet been reported. Here, we generated a new reporter mouse strain, LSL-ACR2 , in which ACR2 is expressed under the control of Cre recombinase. We crossed this strain with a noradrenergic neuron-specific driver mouse ( NAT-Cre ) to generate NAT-ACR2 mice. We confirmed Cre-dependent expression and function of ACR2 in the targeted neurons by immunohistochemistry and electrophysiological recordings in vitro , and confirmed physiological function using an in vivo behavioral experiment. Our results show that the LSL-ACR2 mouse strain can be applied for optogenetic inhibition of targeted neurons, particularly for long-lasting continuous inhibition, upon crossing with Cre-driver mouse strains. The LSL-ACR2 strain can be used to prepare transgenic mice with homogenous expression of ACR2 in targeted neurons with a high penetration ratio, good reproducibility, and no tissue invasion." @default.
- W4313457531 created "2023-01-06" @default.
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- W4313457531 date "2023-01-03" @default.
- W4313457531 modified "2023-10-18" @default.
- W4313457531 title "Cre-dependent ACR2-expressing reporter mouse strain for efficient long-lasting inhibition of neuronal activity" @default.
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- W4313457531 doi "https://doi.org/10.21203/rs.3.rs-2376443/v1" @default.
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