Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313458387> ?p ?o ?g. }
- W4313458387 endingPage "1009" @default.
- W4313458387 startingPage "998" @default.
- W4313458387 abstract "Succinate dehydrogenase inhibitors (SDHIs) are keystone synthetic fungicides used to manage Botrytis cinerea in several hosts. In this study, we investigated the cross-resistance between five new SDHIs (pyraziflumid, isofetamid, benzovindiflupyr, fluxapyroxad, and pydiflumetofen) with commonly used SDHIs boscalid and fluopyram. Different mutations were detected in the sdhB gene in B. cinerea collected from Michigan grapes, and their frequency and EC50 value were determined. Among 216 B. cinerea boscalid-resistant isolates, five different mutations were detected, including H272R/Y, P225F/H, and N230I, at frequencies of 82.6, 4.3, 11.5, 0.4, and 5.3%, respectively. Five isolates of each genotype were used to screen the cross-resistance of the SDHIs. We classified the resistance profile of our mutants into five patterns. We report that all tested mutants were sensitive to benzovindiflupyr, indicating that it can be used as an effective fungicide against all B. cinerea mutants identified in this study. In addition, fluopyram, pydiflumetofen, and isofetamid can provide effective control according to which type of mutation is present in the field. We also developed and compared two molecular diagnostic tools, rhAMP and TaqMan assays, for rapid detection of SDHI resistance-associated mutants in B. cinerea. We report that the TaqMan assay was more successful than the rhAMP assay in detecting the B. cinerea mutant DNA at ≤10 pg and in a single assay was capable of monitoring two amino acid positions. Our results provide essential information about new SDHIs and provide molecular tools for monitoring SDHI resistance mutations, which will assist in gray mold disease control." @default.
- W4313458387 created "2023-01-06" @default.
- W4313458387 creator A5010234659 @default.
- W4313458387 creator A5040383377 @default.
- W4313458387 creator A5050176164 @default.
- W4313458387 creator A5057230847 @default.
- W4313458387 date "2023-06-01" @default.
- W4313458387 modified "2023-10-17" @default.
- W4313458387 title "Cross-Resistance of Succinate Dehydrogenase Inhibitors (SDHI) in <i>Botrytis cinerea</i> and Development of Molecular Diagnostic Tools for SDHI Resistance Detection" @default.
- W4313458387 cites W1483292412 @default.
- W4313458387 cites W1501848088 @default.
- W4313458387 cites W1518565653 @default.
- W4313458387 cites W1891726238 @default.
- W4313458387 cites W1911666230 @default.
- W4313458387 cites W1974708531 @default.
- W4313458387 cites W1979087291 @default.
- W4313458387 cites W1980880841 @default.
- W4313458387 cites W1994349762 @default.
- W4313458387 cites W1999424388 @default.
- W4313458387 cites W2000169208 @default.
- W4313458387 cites W2005375502 @default.
- W4313458387 cites W2035584729 @default.
- W4313458387 cites W2044016099 @default.
- W4313458387 cites W2045484875 @default.
- W4313458387 cites W2052794788 @default.
- W4313458387 cites W2054925635 @default.
- W4313458387 cites W2061117777 @default.
- W4313458387 cites W2069890279 @default.
- W4313458387 cites W2079829462 @default.
- W4313458387 cites W2081703156 @default.
- W4313458387 cites W2091161477 @default.
- W4313458387 cites W2103902328 @default.
- W4313458387 cites W2104042158 @default.
- W4313458387 cites W2112410817 @default.
- W4313458387 cites W2112438801 @default.
- W4313458387 cites W2113377390 @default.
- W4313458387 cites W2113762600 @default.
- W4313458387 cites W2116901274 @default.
- W4313458387 cites W2118751052 @default.
- W4313458387 cites W2128354363 @default.
- W4313458387 cites W2128998083 @default.
- W4313458387 cites W2139456772 @default.
- W4313458387 cites W2155192511 @default.
- W4313458387 cites W2157845086 @default.
- W4313458387 cites W2165730636 @default.
- W4313458387 cites W2188537512 @default.
- W4313458387 cites W2238568022 @default.
- W4313458387 cites W2281447409 @default.
- W4313458387 cites W2285851440 @default.
- W4313458387 cites W2320057050 @default.
- W4313458387 cites W2332639219 @default.
- W4313458387 cites W2340780036 @default.
- W4313458387 cites W2408859044 @default.
- W4313458387 cites W2476864206 @default.
- W4313458387 cites W2551751858 @default.
- W4313458387 cites W2586256562 @default.
- W4313458387 cites W2600369554 @default.
- W4313458387 cites W2605820727 @default.
- W4313458387 cites W2605973074 @default.
- W4313458387 cites W2805232952 @default.
- W4313458387 cites W2809225958 @default.
- W4313458387 cites W2811073389 @default.
- W4313458387 cites W2906899406 @default.
- W4313458387 cites W2946055221 @default.
- W4313458387 cites W2947432633 @default.
- W4313458387 cites W2948573327 @default.
- W4313458387 cites W2952903368 @default.
- W4313458387 cites W2987205532 @default.
- W4313458387 cites W2999255655 @default.
- W4313458387 cites W3008272861 @default.
- W4313458387 cites W3012448237 @default.
- W4313458387 cites W3045289776 @default.
- W4313458387 cites W3047954191 @default.
- W4313458387 cites W3049358869 @default.
- W4313458387 cites W3208945064 @default.
- W4313458387 cites W4200310801 @default.
- W4313458387 cites W4206914148 @default.
- W4313458387 cites W7827006 @default.
- W4313458387 doi "https://doi.org/10.1094/phyto-09-22-0346-r" @default.
- W4313458387 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36596212" @default.
- W4313458387 hasPublicationYear "2023" @default.
- W4313458387 type Work @default.
- W4313458387 citedByCount "1" @default.
- W4313458387 countsByYear W43134583872023 @default.
- W4313458387 crossrefType "journal-article" @default.
- W4313458387 hasAuthorship W4313458387A5010234659 @default.
- W4313458387 hasAuthorship W4313458387A5040383377 @default.
- W4313458387 hasAuthorship W4313458387A5050176164 @default.
- W4313458387 hasAuthorship W4313458387A5057230847 @default.
- W4313458387 hasConcept C104317684 @default.
- W4313458387 hasConcept C143065580 @default.
- W4313458387 hasConcept C143660080 @default.
- W4313458387 hasConcept C179786068 @default.
- W4313458387 hasConcept C2775867217 @default.
- W4313458387 hasConcept C49105822 @default.
- W4313458387 hasConcept C501734568 @default.
- W4313458387 hasConcept C54355233 @default.
- W4313458387 hasConcept C59822182 @default.