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• W4313463478 abstract "ABSTRACT Spectrum library searching is a powerful alternative to database searching for data dependent acquisition experiments, but has been historically limited to identifying previously observed peptides in libraries. Here we present Scribe, a new library search engine designed to leverage deep learning fragmentation prediction software such as Prosit. Rather than relying on highly curated DDA libraries, this approach predicts fragmentation and retention times for every peptide in a FASTA database. Scribe embeds Percolator for FDR correction and an interference tolerant label-free quantification integrator to enable an end-to-end proteomics workflow. By leveraging expected relative fragmentation and retention time values, we find that library searching with Scribe can outperform traditional database searching tools, both in terms of sensitivity and quantitative precision. Scribe and its graphical interface are easy to use, freely accessible, and fully open source." @default.
• W4313463478 created "2023-01-06" @default.
• W4313463478 creator A5010773425 @default.
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• W4313463478 date "2023-01-03" @default.
• W4313463478 modified "2023-10-16" @default.
• W4313463478 title "Scribe: next-generation library searching for DDA experiments" @default.
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• W4313463478 doi "https://doi.org/10.1101/2023.01.01.522445" @default.
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