FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313465510> ?p ?o ?g. }

• W4313465510 abstract "Pichia pastoris is widely used for the production of recombinant proteins, but the low production efficiency hinders its wide application in biopharmaceuticals. Moreover, many biopharmaceutical-like proteins are accompanied by degradation during secretory expression in P. pastoris.In this study, we used human serum albumin and porcine follicle-stimulating hormone β (HSA-pFSHβ) fusion protein as a model protein to investigate whether YPS1 and YPT7 gene disruption and N-acetyl-L-cysteine (NAC) supplementation have synergistic effects to inhibit the degradation of recombinant proteins.Our results showed that YPS1 gene disruption reduced the degradation of intact HSA-pFSHβ and increased the yield of intact protein in the culture medium and cells without affecting the integrity of the cell wall. Moreover, the beneficial effects of YPS1 gene disruption were associated with the upregulation of the MAPK signaling pathway and maintenance of redox homeostasis. YPS1 gene disruption and NAC supplementation had synergistic effects on HSA-pFSHβ production. In addition, disruption of vacuolar morphology by YPT7 gene disruption or NH4Cl treatment affected the production of recombinant HSA-pFSHβ protein. Furthermore, YPT7 gene disruption inhibited the processing of signal peptide in high-level produced HSA-pFSHβ strain. In conclusion, our results demonstrated that YPS1 disruption could reduce the degradation of intact HSA-pFSHβ proteins, and synergistically increase the yield of intact HSA-pFSHβ with NAC supplementation. This study provided a valuable reference for reducing recombinant protein degradation and therefore improving the yield of recombinant proteins in P. pastoris." @default.
• W4313465510 created "2023-01-06" @default.
• W4313465510 creator A5003638381 @default.
• W4313465510 creator A5016942883 @default.
• W4313465510 creator A5022517143 @default.
• W4313465510 creator A5052363873 @default.
• W4313465510 creator A5058122971 @default.
• W4313465510 creator A5068328506 @default.
• W4313465510 creator A5075260509 @default.
• W4313465510 date "2022-12-21" @default.
• W4313465510 modified "2023-10-05" @default.
• W4313465510 title "Enhancement of HSA-pFSHβ production by disrupting YPS1 and supplementing N-acetyl-L-cysteine in Pichia pastoris" @default.
• W4313465510 cites W1598680533 @default.
• W4313465510 cites W1938341712 @default.
• W4313465510 cites W1961697585 @default.
• W4313465510 cites W1975597861 @default.
• W4313465510 cites W1978507010 @default.
• W4313465510 cites W1994528127 @default.
• W4313465510 cites W2007699101 @default.
• W4313465510 cites W2008165835 @default.
• W4313465510 cites W2026701740 @default.
• W4313465510 cites W2027368950 @default.
• W4313465510 cites W2029778591 @default.
• W4313465510 cites W2036896850 @default.
• W4313465510 cites W2046967366 @default.
• W4313465510 cites W2055872607 @default.
• W4313465510 cites W2069643777 @default.
• W4313465510 cites W2077249818 @default.
• W4313465510 cites W2077348717 @default.
• W4313465510 cites W2090509945 @default.
• W4313465510 cites W2092803388 @default.
• W4313465510 cites W2095668363 @default.
• W4313465510 cites W2103491947 @default.
• W4313465510 cites W2119783761 @default.
• W4313465510 cites W2139297116 @default.
• W4313465510 cites W2141747730 @default.
• W4313465510 cites W2141781497 @default.
• W4313465510 cites W2142351659 @default.
• W4313465510 cites W2142621741 @default.
• W4313465510 cites W2148009678 @default.
• W4313465510 cites W2150610841 @default.
• W4313465510 cites W2154087334 @default.
• W4313465510 cites W2155333588 @default.
• W4313465510 cites W2158501357 @default.
• W4313465510 cites W2160317147 @default.
• W4313465510 cites W2319904471 @default.
• W4313465510 cites W2547805125 @default.
• W4313465510 cites W2591544263 @default.
• W4313465510 cites W2886165156 @default.
• W4313465510 cites W2888280327 @default.
• W4313465510 cites W2907751315 @default.
• W4313465510 cites W2914196574 @default.
• W4313465510 cites W2917973694 @default.
• W4313465510 cites W2955558730 @default.
• W4313465510 cites W2968472275 @default.
• W4313465510 cites W2989756115 @default.
• W4313465510 cites W3005701149 @default.
• W4313465510 cites W3012063587 @default.
• W4313465510 cites W3097867614 @default.
• W4313465510 cites W3123705305 @default.
• W4313465510 cites W3183224575 @default.
• W4313465510 cites W3197835005 @default.
• W4313465510 cites W4213002627 @default.
• W4313465510 doi "https://doi.org/10.3389/fmicb.2022.998647" @default.
• W4313465510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36620033" @default.
• W4313465510 hasPublicationYear "2022" @default.
• W4313465510 type Work @default.
• W4313465510 citedByCount "1" @default.
• W4313465510 countsByYear W43134655102023 @default.
• W4313465510 crossrefType "journal-article" @default.
• W4313465510 hasAuthorship W4313465510A5003638381 @default.
• W4313465510 hasAuthorship W4313465510A5016942883 @default.
• W4313465510 hasAuthorship W4313465510A5022517143 @default.
• W4313465510 hasAuthorship W4313465510A5052363873 @default.
• W4313465510 hasAuthorship W4313465510A5058122971 @default.
• W4313465510 hasAuthorship W4313465510A5068328506 @default.
• W4313465510 hasAuthorship W4313465510A5075260509 @default.
• W4313465510 hasBestOaLocation W43134655101 @default.
• W4313465510 hasConcept C104317684 @default.
• W4313465510 hasConcept C156399914 @default.
• W4313465510 hasConcept C2781372317 @default.
• W4313465510 hasConcept C3675279 @default.
• W4313465510 hasConcept C40767141 @default.
• W4313465510 hasConcept C55493867 @default.
• W4313465510 hasConcept C86803240 @default.
• W4313465510 hasConcept C95444343 @default.
• W4313465510 hasConceptScore W4313465510C104317684 @default.
• W4313465510 hasConceptScore W4313465510C156399914 @default.
• W4313465510 hasConceptScore W4313465510C2781372317 @default.
• W4313465510 hasConceptScore W4313465510C3675279 @default.
• W4313465510 hasConceptScore W4313465510C40767141 @default.
• W4313465510 hasConceptScore W4313465510C55493867 @default.
• W4313465510 hasConceptScore W4313465510C86803240 @default.
• W4313465510 hasConceptScore W4313465510C95444343 @default.
• W4313465510 hasFunder F4320321001 @default.
• W4313465510 hasLocation W43134655101 @default.
• W4313465510 hasLocation W43134655102 @default.
• W4313465510 hasLocation W43134655103 @default.
• W4313465510 hasOpenAccess W4313465510 @default.
• W4313465510 hasPrimaryLocation W43134655101 @default.

• next