Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313466427> ?p ?o ?g. }
- W4313466427 endingPage "833" @default.
- W4313466427 startingPage "833" @default.
- W4313466427 abstract "Iron-sulfur (Fe-S) clusters are essential cofactors for enzyme activity. These Fe-S clusters are present in structurally diverse forms, including [4Fe-4S] and [3Fe-4S]. Type-identification of the Fe-S cluster is indispensable in understanding the catalytic mechanism of enzymes. However, identifying [4Fe-4S] and [3Fe-4S] clusters in particular is challenging because of their rapid transformation in response to oxidation-reduction events. In this study, we focused on the relationship between the Fe-S cluster type and the catalytic activity of a tRNA-thiolation enzyme (TtuA). We reconstituted [4Fe-4S]-TtuA, prepared [3Fe-4S]-TtuA by oxidizing [4Fe-4S]-TtuA under strictly anaerobic conditions, and then observed changes in the Fe-S clusters in the samples and the enzymatic activity in the time-course experiments. Electron paramagnetic resonance analysis revealed that [3Fe-4S]-TtuA spontaneously transforms into [4Fe-4S]-TtuA in minutes to one hour without an additional free Fe source in the solution. Although the TtuA immediately after oxidation of [4Fe-4S]-TtuA was inactive [3Fe-4S]-TtuA, its activity recovered to a significant level compared to [4Fe-4S]-TtuA after one hour, corresponding to an increase of [4Fe-4S]-TtuA in the solution. Our findings reveal that [3Fe-4S]-TtuA is highly inactive and unstable. Moreover, time-course analysis of structural changes and activity under strictly anaerobic conditions further unraveled the Fe-S cluster type used by the tRNA-thiolation enzyme." @default.
- W4313466427 created "2023-01-06" @default.
- W4313466427 creator A5016535718 @default.
- W4313466427 creator A5022233637 @default.
- W4313466427 creator A5064202029 @default.
- W4313466427 creator A5065748566 @default.
- W4313466427 creator A5072020826 @default.
- W4313466427 creator A5074927675 @default.
- W4313466427 creator A5077307889 @default.
- W4313466427 date "2023-01-03" @default.
- W4313466427 modified "2023-10-14" @default.
- W4313466427 title "Quick and Spontaneous Transformation between [3Fe–4S] and [4Fe–4S] Iron–Sulfur Clusters in the tRNA-Thiolation Enzyme TtuA" @default.
- W4313466427 cites W1505447146 @default.
- W4313466427 cites W1544860062 @default.
- W4313466427 cites W1972318763 @default.
- W4313466427 cites W1974719543 @default.
- W4313466427 cites W1981376769 @default.
- W4313466427 cites W1985797027 @default.
- W4313466427 cites W1986316905 @default.
- W4313466427 cites W1994129311 @default.
- W4313466427 cites W2012020430 @default.
- W4313466427 cites W2016906651 @default.
- W4313466427 cites W2023733301 @default.
- W4313466427 cites W2033367616 @default.
- W4313466427 cites W2040118814 @default.
- W4313466427 cites W2076980917 @default.
- W4313466427 cites W2084744164 @default.
- W4313466427 cites W2086848382 @default.
- W4313466427 cites W2090079874 @default.
- W4313466427 cites W2094514994 @default.
- W4313466427 cites W2095724872 @default.
- W4313466427 cites W2107090468 @default.
- W4313466427 cites W2120269671 @default.
- W4313466427 cites W2128635872 @default.
- W4313466427 cites W2139891610 @default.
- W4313466427 cites W2146684384 @default.
- W4313466427 cites W2149528769 @default.
- W4313466427 cites W2151474499 @default.
- W4313466427 cites W2152702717 @default.
- W4313466427 cites W2168974130 @default.
- W4313466427 cites W2170476506 @default.
- W4313466427 cites W2396643508 @default.
- W4313466427 cites W2533780185 @default.
- W4313466427 cites W2557220845 @default.
- W4313466427 cites W2608122210 @default.
- W4313466427 cites W2725127376 @default.
- W4313466427 cites W2896007459 @default.
- W4313466427 cites W2992797755 @default.
- W4313466427 cites W3014419632 @default.
- W4313466427 cites W3017173206 @default.
- W4313466427 cites W3042177030 @default.
- W4313466427 cites W3138714128 @default.
- W4313466427 cites W3164001450 @default.
- W4313466427 cites W3177828909 @default.
- W4313466427 cites W3209202387 @default.
- W4313466427 cites W3213389863 @default.
- W4313466427 cites W3215591662 @default.
- W4313466427 cites W4200307180 @default.
- W4313466427 cites W4293247451 @default.
- W4313466427 doi "https://doi.org/10.3390/ijms24010833" @default.
- W4313466427 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36614280" @default.
- W4313466427 hasPublicationYear "2023" @default.
- W4313466427 type Work @default.
- W4313466427 citedByCount "0" @default.
- W4313466427 crossrefType "journal-article" @default.
- W4313466427 hasAuthorship W4313466427A5016535718 @default.
- W4313466427 hasAuthorship W4313466427A5022233637 @default.
- W4313466427 hasAuthorship W4313466427A5064202029 @default.
- W4313466427 hasAuthorship W4313466427A5065748566 @default.
- W4313466427 hasAuthorship W4313466427A5072020826 @default.
- W4313466427 hasAuthorship W4313466427A5074927675 @default.
- W4313466427 hasAuthorship W4313466427A5077307889 @default.
- W4313466427 hasBestOaLocation W43134664271 @default.
- W4313466427 hasConcept C104317684 @default.
- W4313466427 hasConcept C121332964 @default.
- W4313466427 hasConcept C123669783 @default.
- W4313466427 hasConcept C161790260 @default.
- W4313466427 hasConcept C164866538 @default.
- W4313466427 hasConcept C178790620 @default.
- W4313466427 hasConcept C179104552 @default.
- W4313466427 hasConcept C181199279 @default.
- W4313466427 hasConcept C185592680 @default.
- W4313466427 hasConcept C187961010 @default.
- W4313466427 hasConcept C197957613 @default.
- W4313466427 hasConcept C199360897 @default.
- W4313466427 hasConcept C204241405 @default.
- W4313466427 hasConcept C2777852564 @default.
- W4313466427 hasConcept C41008148 @default.
- W4313466427 hasConcept C46141821 @default.
- W4313466427 hasConcept C518881349 @default.
- W4313466427 hasConcept C55493867 @default.
- W4313466427 hasConcept C55904794 @default.
- W4313466427 hasConcept C75473681 @default.
- W4313466427 hasConcept C77176794 @default.
- W4313466427 hasConcept C8010536 @default.
- W4313466427 hasConceptScore W4313466427C104317684 @default.
- W4313466427 hasConceptScore W4313466427C121332964 @default.
- W4313466427 hasConceptScore W4313466427C123669783 @default.