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• W4313468640 startingPage "9" @default.
• W4313468640 abstract "The aim of this study was to isolate phage enzymes and apply them in vitro for eradication of the dominant saprophytic bacteria isolated from minimally processed food. Four bacteriophages-two Enterobacter-specific and two Serratia-specific, which produce lytic enzymes-were used in this research. Two methods of phage enzyme isolation were tested, namely precipitation with acetone and ultracentrifugation. It was found that the number of virions could be increased almost 100 times due to the extension of the cultivation time (72 h). The amplification of phage particles and lytic proteins was dependent on the time of cultivation. Considering the influence of isolated enzymes on the growth kinetics of bacterial hosts, proteins isolated with acetone after 72-hour phage propagation exhibited the highest inhibitory effect. The reduction of bacteria count was dependent on the concentration of enzymes in the lysates. The obtained results indicate that phages and their lytic enzymes could be used in further research aiming at the improvement of microbiological quality and safety of minimally processed food products." @default.
• W4313468640 created "2023-01-06" @default.
• W4313468640 creator A5014962647 @default.
• W4313468640 creator A5022506039 @default.
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• W4313468640 creator A5058453188 @default.
• W4313468640 creator A5078175050 @default.
• W4313468640 creator A5088330912 @default.
• W4313468640 date "2022-12-20" @default.
• W4313468640 modified "2023-09-26" @default.
• W4313468640 title "Application of Lytic Bacteriophages and Their Enzymes to Reduce Saprophytic Bacteria Isolated from Minimally Processed Plant-Based Food Products—In Vitro Studies" @default.
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