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- W4313471206 endingPage "132" @default.
- W4313471206 startingPage "132" @default.
- W4313471206 abstract "The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells." @default.
- W4313471206 created "2023-01-06" @default.
- W4313471206 creator A5008159170 @default.
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- W4313471206 creator A5068302332 @default.
- W4313471206 creator A5081396029 @default.
- W4313471206 date "2022-12-31" @default.
- W4313471206 modified "2023-10-18" @default.
- W4313471206 title "Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells" @default.
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- W4313471206 doi "https://doi.org/10.3390/v15010132" @default.
- W4313471206 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36680173" @default.
- W4313471206 hasPublicationYear "2022" @default.
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