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- W4313485247 abstract "To be effective, microbiological studies of deep aquifers must be free from surface microbial contaminants and from infrastructures allowing access to formation water (wellheads, well completions). Many microbiological studies are based on water samples obtained after rinsing a well without guaranteeing the absence of contaminants from the biofilm development in the pipes. The protocol described in this paper presents the adaptation, preparation, sterilization and deployment of a commercial downhole sampler (PDSshort, Leutert, Germany) for the microbiological studying of deep aquifers. The ATEX sampler (i.e., explosive atmospheres) can be deployed for geological gas storage (methane, hydrogen). To validate our procedure and confirm the need to use such a device, cell counting and bacterial taxonomic diversity based on high-throughput sequencing for different water samples taken at the wellhead or at depth using the downhole sampler were compared and discussed. The results show that even after extensive rinsing (7 bore volumes), the water collected at the wellhead was not free of microbial contaminants, as shown by beta-diversity analysis. The downhole sampler procedure was the only way to ensure the purity of the formation water samples from the microbiological point of view. In addition, the downhole sampler allowed the formation water and the autochthonous microbial community to be maintained at in situ pressure for laboratory analysis. The prevention of the contamination of the sample and the preservation of its representativeness are key to guaranteeing the best interpretations and understanding of the functioning of the deep biosphere." @default.
- W4313485247 created "2023-01-06" @default.
- W4313485247 creator A5022314202 @default.
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- W4313485247 date "2023-01-04" @default.
- W4313485247 modified "2023-10-12" @default.
- W4313485247 title "A deep continental aquifer downhole sampler for microbiological studies" @default.
- W4313485247 cites W1531078984 @default.
- W4313485247 cites W1656962512 @default.
- W4313485247 cites W1922601076 @default.
- W4313485247 cites W1929744349 @default.
- W4313485247 cites W1960582054 @default.
- W4313485247 cites W1977618577 @default.
- W4313485247 cites W1990826869 @default.
- W4313485247 cites W1995869732 @default.
- W4313485247 cites W2002203597 @default.
- W4313485247 cites W2005497307 @default.
- W4313485247 cites W2006401467 @default.
- W4313485247 cites W2010438276 @default.
- W4313485247 cites W2010615882 @default.
- W4313485247 cites W2010812271 @default.
- W4313485247 cites W2011422030 @default.
- W4313485247 cites W2014879763 @default.
- W4313485247 cites W2016852233 @default.
- W4313485247 cites W2028145994 @default.
- W4313485247 cites W2029472479 @default.
- W4313485247 cites W2031227761 @default.
- W4313485247 cites W2031863726 @default.
- W4313485247 cites W2033242541 @default.
- W4313485247 cites W2034431434 @default.
- W4313485247 cites W2038132750 @default.
- W4313485247 cites W2039257492 @default.
- W4313485247 cites W2044933285 @default.
- W4313485247 cites W2047477530 @default.
- W4313485247 cites W2056279562 @default.
- W4313485247 cites W2060894986 @default.
- W4313485247 cites W2110777357 @default.
- W4313485247 cites W2111364030 @default.
- W4313485247 cites W2113071589 @default.
- W4313485247 cites W2132193068 @default.
- W4313485247 cites W2133377589 @default.
- W4313485247 cites W2134544457 @default.
- W4313485247 cites W2136541274 @default.
- W4313485247 cites W2136879569 @default.
- W4313485247 cites W2140448721 @default.
- W4313485247 cites W2143563342 @default.
- W4313485247 cites W2151121517 @default.
- W4313485247 cites W2154401636 @default.
- W4313485247 cites W2157107905 @default.
- W4313485247 cites W2160209604 @default.
- W4313485247 cites W2168381925 @default.
- W4313485247 cites W2261399118 @default.
- W4313485247 cites W2295587945 @default.
- W4313485247 cites W2510331270 @default.
- W4313485247 cites W2513506562 @default.
- W4313485247 cites W2537305183 @default.
- W4313485247 cites W2547684158 @default.
- W4313485247 cites W2548488054 @default.
- W4313485247 cites W2573133525 @default.
- W4313485247 cites W2582543953 @default.
- W4313485247 cites W2587757245 @default.
- W4313485247 cites W2621290343 @default.
- W4313485247 cites W2736099625 @default.
- W4313485247 cites W2762087089 @default.
- W4313485247 cites W2765241592 @default.
- W4313485247 cites W2767960552 @default.
- W4313485247 cites W2769268519 @default.
- W4313485247 cites W2774551105 @default.
- W4313485247 cites W2781915920 @default.
- W4313485247 cites W2889102481 @default.
- W4313485247 cites W2891788358 @default.
- W4313485247 cites W2894440084 @default.
- W4313485247 cites W2900606268 @default.
- W4313485247 cites W2903169084 @default.
- W4313485247 cites W2940456019 @default.
- W4313485247 cites W2959042974 @default.
- W4313485247 cites W2959816311 @default.
- W4313485247 cites W2968272598 @default.
- W4313485247 cites W2972976523 @default.
- W4313485247 cites W2977284782 @default.
- W4313485247 cites W2988774311 @default.
- W4313485247 cites W2995795907 @default.
- W4313485247 cites W3006090474 @default.
- W4313485247 cites W3023877562 @default.
- W4313485247 cites W3039192999 @default.
- W4313485247 cites W3081871794 @default.
- W4313485247 cites W3099018631 @default.
- W4313485247 cites W3128016313 @default.
- W4313485247 cites W3159947045 @default.
- W4313485247 cites W3204607296 @default.