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- W4313489030 abstract "Bordetella pertussis causes whooping cough and is transmitted via respiratory droplets. Here, we present a protocol to challenge mice with Bordetella pertussis. We describe bacteria preparation and long-term storage, followed by manufacturing a challenge dose for use in a commercial exposure chamber with controlled nebulization of B. pertussis into aerosols. We then detail the aerosol challenge of mice through a more natural administration than intranasal instillation and post-challenge data collection. This protocol allows for better comparisons between preclinical pertussis studies." @default.
- W4313489030 created "2023-01-06" @default.
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- W4313489030 date "2023-03-01" @default.
- W4313489030 modified "2023-10-01" @default.
- W4313489030 title "Protocol for aerosolization challenge of mice with Bordetella pertussis" @default.
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- W4313489030 doi "https://doi.org/10.1016/j.xpro.2022.101979" @default.
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