FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313489216> ?p ?o ?g. }

• W4313489216 abstract "During yeast stationary phase, a single spherical vacuole (lysosome) is created by the fusion of several small ones. Moreover, the vacuolar membrane is reconstructed into two distinct microdomains. Little is known, however, about how cells maintain vacuolar shape or regulate their microdomains. Here, we show that Fat1p, a fatty acyl coenzyme A (acyl-CoA) synthetase and fatty acid transporter, and not the synthetases Faa1p and Faa4p, is essential for vacuolar shape preservation, the development of vacuolar microdomains, and cell survival in stationary phase of the yeast Saccharomyces cerevisiae. Furthermore, Fat1p negatively regulates general autophagy in both log- and stationary-phase cells. In contrast, Fat1p promotes lipophagy, as the absence of FAT1 limits the entry of lipid droplets into the vacuole and reduces the degradation of liquid droplet (LD) surface proteins. Notably, supplementing with unsaturated fatty acids or overexpressing the desaturase Ole1p can reverse all aberrant phenotypes caused by FAT1 deficiency. We propose that Fat1p regulates stationary phase vacuolar morphology, microdomain differentiation, general autophagy, and lipophagy by controlling the degree of fatty acid saturation in membrane lipids. IMPORTANCE The ability to sense environmental changes and adjust the levels of cellular metabolism is critical for cell viability. Autophagy is a recycling process that makes the most of already-existing energy resources, and the vacuole/lysosome is the ultimate autophagic processing site in cells. Lipophagy is an autophagic process to select degrading lipid droplets. In yeast cells in stationary phase, vacuoles fuse and remodel their membranes to create a single spherical vacuole with two distinct membrane microdomains, which are required for yeast lipophagy. In this study, we discovered that Fat1p was capable of rapidly responding to changes in nutritional status and preserving cell survival by regulating membrane lipid saturation to maintain proper vacuolar morphology and the level of lipophagy in the yeast S. cerevisiae. Our findings shed light on how cells maintain vacuolar structure and promote the differentiation of vacuole surface microdomains for stationary-phase lipophagy." @default.
• W4313489216 created "2023-01-06" @default.
• W4313489216 creator A5041388998 @default.
• W4313489216 creator A5049507916 @default.
• W4313489216 creator A5059689012 @default.
• W4313489216 creator A5066644794 @default.
• W4313489216 creator A5066837275 @default.
• W4313489216 creator A5073898745 @default.
• W4313489216 creator A5086090361 @default.
• W4313489216 creator A5091269556 @default.
• W4313489216 date "2023-02-14" @default.
• W4313489216 modified "2023-10-14" @default.
• W4313489216 title "Fatty Acyl Coenzyme A Synthetase Fat1p Regulates Vacuolar Structure and Stationary-Phase Lipophagy in Saccharomyces cerevisiae" @default.
• W4313489216 cites W1482852045 @default.
• W4313489216 cites W1502020403 @default.
• W4313489216 cites W1588738313 @default.
• W4313489216 cites W1754519657 @default.
• W4313489216 cites W1967513256 @default.
• W4313489216 cites W1968917541 @default.
• W4313489216 cites W1972950841 @default.
• W4313489216 cites W1980783399 @default.
• W4313489216 cites W1990589135 @default.
• W4313489216 cites W1993777075 @default.
• W4313489216 cites W2000961724 @default.
• W4313489216 cites W2006023613 @default.
• W4313489216 cites W2007505836 @default.
• W4313489216 cites W2009128541 @default.
• W4313489216 cites W2012118159 @default.
• W4313489216 cites W2021919397 @default.
• W4313489216 cites W2028215693 @default.
• W4313489216 cites W2034911317 @default.
• W4313489216 cites W2035327550 @default.
• W4313489216 cites W2044480313 @default.
• W4313489216 cites W2045689338 @default.
• W4313489216 cites W2047458976 @default.
• W4313489216 cites W2049527119 @default.
• W4313489216 cites W2053519558 @default.
• W4313489216 cites W2056478340 @default.
• W4313489216 cites W2056633943 @default.
• W4313489216 cites W2066849342 @default.
• W4313489216 cites W2068275754 @default.
• W4313489216 cites W2068749469 @default.
• W4313489216 cites W2072405906 @default.
• W4313489216 cites W2082800491 @default.
• W4313489216 cites W2083589342 @default.
• W4313489216 cites W2083727519 @default.
• W4313489216 cites W2087250756 @default.
• W4313489216 cites W2091896084 @default.
• W4313489216 cites W2093223217 @default.
• W4313489216 cites W2099538852 @default.
• W4313489216 cites W2105821361 @default.
• W4313489216 cites W2106020452 @default.
• W4313489216 cites W2110522042 @default.
• W4313489216 cites W2110898186 @default.
• W4313489216 cites W2112520822 @default.
• W4313489216 cites W2118057509 @default.
• W4313489216 cites W2121233961 @default.
• W4313489216 cites W2125452667 @default.
• W4313489216 cites W2129326001 @default.
• W4313489216 cites W2133212160 @default.
• W4313489216 cites W2141747730 @default.
• W4313489216 cites W2157785265 @default.
• W4313489216 cites W2162781372 @default.
• W4313489216 cites W2163861471 @default.
• W4313489216 cites W2169333620 @default.
• W4313489216 cites W2204033497 @default.
• W4313489216 cites W2278612953 @default.
• W4313489216 cites W2293141975 @default.
• W4313489216 cites W2622808466 @default.
• W4313489216 cites W2754646635 @default.
• W4313489216 cites W2758797028 @default.
• W4313489216 cites W2790360897 @default.
• W4313489216 cites W2795845448 @default.
• W4313489216 cites W2801949225 @default.
• W4313489216 cites W2883158359 @default.
• W4313489216 cites W2891163401 @default.
• W4313489216 cites W2906401896 @default.
• W4313489216 cites W2917759590 @default.
• W4313489216 cites W2989581073 @default.
• W4313489216 cites W2997832844 @default.
• W4313489216 cites W3006421071 @default.
• W4313489216 cites W3092204794 @default.
• W4313489216 cites W3095081457 @default.
• W4313489216 cites W3109058671 @default.
• W4313489216 cites W3176483927 @default.
• W4313489216 cites W3176952120 @default.
• W4313489216 cites W3194078705 @default.
• W4313489216 cites W3211928633 @default.
• W4313489216 cites W4206381919 @default.
• W4313489216 cites W4226072802 @default.
• W4313489216 cites W4376848309 @default.
• W4313489216 cites W3145949091 @default.
• W4313489216 doi "https://doi.org/10.1128/spectrum.04625-22" @default.
• W4313489216 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36598223" @default.
• W4313489216 hasPublicationYear "2023" @default.
• W4313489216 type Work @default.
• W4313489216 citedByCount "0" @default.
• W4313489216 crossrefType "journal-article" @default.
• W4313489216 hasAuthorship W4313489216A5041388998 @default.
• W4313489216 hasAuthorship W4313489216A5049507916 @default.

• next