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- W4313506578 abstract "Tannase or tannin acyl hydrolase (EC. 3.1.120) has diverse potential applications in the food industry, because of this, it is necessary to count with different versions of the enzyme. Microbial origin enzymes have acceptance in the biotech industry. Moreover, metagenomics has opened new paths of investigation by allowing analysis without precedents of genome heterogeneity and uncultivable microorganisms. This study was done under the following objectives: (1) to amplify the Tannase gene from metagenomic organisms from soil of Coahuila locations with extreme environments; and (2) to determine the variability of the tannase gene in metagenomic samples. Soils of Cuatro Ciénegas, Ojo Caliente, La Azufrosa, Paila and Dunas de Viesca, Coahuila, México were sampled. Metagenomic DNA was isolated from these soils using methods based on CTAB (cetyl trimethylammonium bromide) and TENS. PCR was run using specific primers to amplified a segment of the tannase gene, and the variability if this gene section was determined using the DGGE technique. The results indicated that amplification of tannase gene from seven soil samples from Ojo Caliente, two from Paila, three from Dunas de Viesca, two from Cuatro Ciénegas and two from La Azufrosa was accomplished, noting the greatest variability in samples 2 and 3 of Ojo Caliente with the highest number of alleles. Whereas the most distant according to the allele variants that the tannase gene possesses, were samples 11 and 15 of Dunas de Viesca and La Azufrosa, respectively. This suggests that in the semi-desert region of Mexico presence of metagenomics organisms with the tannase gene exist and that the same gene presents a variability of alleles." @default.
- W4313506578 created "2023-01-06" @default.
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- W4313506578 date "2023-01-01" @default.
- W4313506578 modified "2023-10-17" @default.
- W4313506578 title "Variability of the Tannase Gene from Extreme Environments Uncultivable Microorganisms" @default.
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- W4313506578 doi "https://doi.org/10.1007/978-981-19-4336-2_19" @default.
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