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• W4313531350 endingPage "100154" @default.
• W4313531350 startingPage "100154" @default.
• W4313531350 abstract "As protein-based therapeutics often exhibit a limited stability in liquid formulations, there is a growing interest in the development of solid protein formulations due to improved protein stability in the solid state. We used small-scale (<3 g) ram and twin-screw extrusion for the solid stabilization of proteins (Lysozyme, BSA, and human insulin) in PEG-matrices. Protein stability after extrusion was systematically investigated using ss-DSC, ss-FTIR, CD spectroscopy, SEM-EDX, SEC, RP-HPLC, and in case of Lysozyme an activity assay. The applied analytical methods offered an accurate assessment of protein stability in extrudates, enabling the comparison of different melt extrusion formulations and process parameters (e.g., shear stress levels, screw configurations, residence times). Lysozyme was implemented as a model protein and was completely recovered in its active form after extrusion. Differences seen between Lysozyme- and BSA- or human insulin-loaded extrudates indicated that melt extrusion could have an impact on the conformational stability. In particular, BSA and human insulin were more susceptible to heat exposure and shear stress compared to Lysozyme, where shear stress was the dominant parameter. Consequently, ram extrusion led to less conformational changes compared to TSE. Ram extrusion showed good protein particle distribution resulting in the preferred method to prepare highly-loaded solid protein formulations." @default.
• W4313531350 created "2023-01-06" @default.
• W4313531350 creator A5025969327 @default.
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• W4313531350 date "2023-12-01" @default.
• W4313531350 modified "2023-09-30" @default.
• W4313531350 title "Impact of process stress on protein stability in highly-loaded solid protein/PEG formulations from small-scale melt extrusion" @default.
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• W4313531350 doi "https://doi.org/10.1016/j.ijpx.2022.100154" @default.
• W4313531350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36632069" @default.
• W4313531350 hasPublicationYear "2023" @default.
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