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- W4313546564 abstract "Several light-inducible hetero-dimerization tools have been developed to spatiotemporally control subcellular localization and activity of target proteins or their downstream signaling. In contrast to other genetic technologies, such as CRISPR-mediated genome editing, these optogenetic tools can locally control protein localization on the second timescale. In addition, these tools can be used to understand the sufficiency of target proteins’ function and manipulate downstream events. In this chapter, I will present methods for locally activating cytoplasmic dynein at the mitotic cell cortex in human cells, with a focus on how to generate knock-in cell lines and set up a microscope system." @default.
- W4313546564 created "2023-01-06" @default.
- W4313546564 creator A5064426475 @default.
- W4313546564 date "2023-01-01" @default.
- W4313546564 modified "2023-09-23" @default.
- W4313546564 title "Using Optogenetics to Spatially Control Cortical Dynein Activity in Mitotic Human Cells" @default.
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- W4313546564 doi "https://doi.org/10.1007/978-1-0716-2958-1_5" @default.
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