FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313552002> ?p ?o ?g. }

• W4313552002 endingPage "e102" @default.
• W4313552002 startingPage "e95" @default.
• W4313552002 abstract "Background Diospyros peregrina is dioecious plant native to India and belonging to the family of Ebenaceae, is largely utilized in treatment of various ailments. Little has been known about the antitumor activity of Diospyros peregrina with only 1 previous study on Ehrlich Ascites Carcinoma in mice. Therefore, it prompted us to extensively explore the immunomodulatory effect in various cancer forms. The focal point of this study revolves around breast cancer, which is the second most common cancer in the world. In view of the increasing demands for noninvasive treatments, natural plant-based agents open up promising applications in cancer immunotherapy Methods CD4+ lymphocytes were isolated from the peripheral blood mononuclear cells (PBMCs) of breast cancer patients and normal donor blood samples using magnetic-activated cell sorting (MACS) and cultured separately. Utilizing the plastic surface adherence property, the macrophages were isolated from CD4 negative lymphocytes of both breast cancer patients and normal donors. For the presentation of tumor antigens invitro, macrophages were pulsed with breast tumor associated antigen (BTAA) in presence or absence of Diospyros peregrina fruit preparation (DFP). Differentially pulsed and irradiated macrophages were co-cultured with autologous and allogenic lymphocytes. Supernatants hence collected from CD4+ lymphocytes were utilized for cytokine profiling using ELISA and proliferation was assessed by MTT assay. Cytotoxic T lymphocytes (CTLs) generated from CD4 negative lymphocytes culture (2 × 105) was incubated with MCF-7 (2 × 104) to check cytotoxicity using LDH release assay. CD4+ lymphocytes were treated in presence or absence of DFP, were analyzed using immunoblotting and RT-qPCR, to check DFP mediated T helper (Th) cell differentiation through investigation of signatory cytokines and transcription factors. Results It was found that DFP elevated the proliferation of CD4+ T lymphocytes (Th) in response to BTAA. DFP also helped in presenting BTAA pulsed macrophages directing in the cytotoxic T-lymphocyte mediated immune response. Results indicated that DFP preferentially highlighted Th1 commitment with type-1 specific cytokines IFN-g and IL-12 and was indifferent in Th2 manifestation. DFP was not only involved in the upregulation of Tbet mounted type-1 mediated immune response and activation of STAT1 but also it downregulated STAT6 and GATA3, the functional activators and regulators of type-2 immune response. Moreover, it was observed that DFP inhibited the tumor-promoting environment modulated through Tregs by downregulating Foxp3 and STAT5. Further, it was detected that DFP directs Th1 bias and results in attainment of better suppression of breast tumor Conclusion The results collectively pointed out that DFP favored cell-mediated immune response from BTAA antigen presentation on macrophages and also helping in the robust proliferation of an entire spectrum of T helper lymphocytes which furthermore strengthen the underlying immune responses, hence, fencing the body, of the progression of breast cancer." @default.
• W4313552002 created "2023-01-06" @default.
• W4313552002 creator A5006619613 @default.
• W4313552002 creator A5032127018 @default.
• W4313552002 creator A5035167563 @default.
• W4313552002 creator A5039124615 @default.
• W4313552002 creator A5080809970 @default.
• W4313552002 date "2023-04-01" @default.
• W4313552002 modified "2023-09-23" @default.
• W4313552002 title "Immunomodulatory Role of Diospyros peregrina Fruit Preparation in Breast Cancer by Utilizing Macrophage Mediated Antigen Presentation and T Helper Cell (Th) Differentiation" @default.
• W4313552002 cites W1761476131 @default.
• W4313552002 cites W1913340502 @default.
• W4313552002 cites W1966771750 @default.
• W4313552002 cites W1967939624 @default.
• W4313552002 cites W1971085539 @default.
• W4313552002 cites W1976324953 @default.
• W4313552002 cites W1983516942 @default.
• W4313552002 cites W1996732951 @default.
• W4313552002 cites W2015280752 @default.
• W4313552002 cites W2015619258 @default.
• W4313552002 cites W2030013787 @default.
• W4313552002 cites W2041724121 @default.
• W4313552002 cites W2051242210 @default.
• W4313552002 cites W2084936411 @default.
• W4313552002 cites W2108765339 @default.
• W4313552002 cites W2112400097 @default.
• W4313552002 cites W2119767265 @default.
• W4313552002 cites W2412475274 @default.
• W4313552002 cites W2511169567 @default.
• W4313552002 cites W2772629056 @default.
• W4313552002 cites W2809740307 @default.
• W4313552002 cites W2889646458 @default.
• W4313552002 cites W2902905881 @default.
• W4313552002 cites W2921191661 @default.
• W4313552002 doi "https://doi.org/10.1016/j.clbc.2022.12.020" @default.
• W4313552002 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36641322" @default.
• W4313552002 hasPublicationYear "2023" @default.
• W4313552002 type Work @default.
• W4313552002 citedByCount "0" @default.
• W4313552002 crossrefType "journal-article" @default.
• W4313552002 hasAuthorship W4313552002A5006619613 @default.
• W4313552002 hasAuthorship W4313552002A5032127018 @default.
• W4313552002 hasAuthorship W4313552002A5035167563 @default.
• W4313552002 hasAuthorship W4313552002A5039124615 @default.
• W4313552002 hasAuthorship W4313552002A5080809970 @default.
• W4313552002 hasConcept C121608353 @default.
• W4313552002 hasConcept C126322002 @default.
• W4313552002 hasConcept C137061746 @default.
• W4313552002 hasConcept C147483822 @default.
• W4313552002 hasConcept C154317977 @default.
• W4313552002 hasConcept C202751555 @default.
• W4313552002 hasConcept C203014093 @default.
• W4313552002 hasConcept C2776662205 @default.
• W4313552002 hasConcept C2777701055 @default.
• W4313552002 hasConcept C2779859075 @default.
• W4313552002 hasConcept C502942594 @default.
• W4313552002 hasConcept C530470458 @default.
• W4313552002 hasConcept C55493867 @default.
• W4313552002 hasConcept C59822182 @default.
• W4313552002 hasConcept C71924100 @default.
• W4313552002 hasConcept C86803240 @default.
• W4313552002 hasConcept C8891405 @default.
• W4313552002 hasConceptScore W4313552002C121608353 @default.
• W4313552002 hasConceptScore W4313552002C126322002 @default.
• W4313552002 hasConceptScore W4313552002C137061746 @default.
• W4313552002 hasConceptScore W4313552002C147483822 @default.
• W4313552002 hasConceptScore W4313552002C154317977 @default.
• W4313552002 hasConceptScore W4313552002C202751555 @default.
• W4313552002 hasConceptScore W4313552002C203014093 @default.
• W4313552002 hasConceptScore W4313552002C2776662205 @default.
• W4313552002 hasConceptScore W4313552002C2777701055 @default.
• W4313552002 hasConceptScore W4313552002C2779859075 @default.
• W4313552002 hasConceptScore W4313552002C502942594 @default.
• W4313552002 hasConceptScore W4313552002C530470458 @default.
• W4313552002 hasConceptScore W4313552002C55493867 @default.
• W4313552002 hasConceptScore W4313552002C59822182 @default.
• W4313552002 hasConceptScore W4313552002C71924100 @default.
• W4313552002 hasConceptScore W4313552002C86803240 @default.
• W4313552002 hasConceptScore W4313552002C8891405 @default.
• W4313552002 hasFunder F4320320719 @default.
• W4313552002 hasFunder F4320334771 @default.
• W4313552002 hasIssue "3" @default.
• W4313552002 hasLocation W43135520021 @default.
• W4313552002 hasLocation W43135520022 @default.
• W4313552002 hasOpenAccess W4313552002 @default.
• W4313552002 hasPrimaryLocation W43135520021 @default.
• W4313552002 hasRelatedWork W1540201991 @default.
• W4313552002 hasRelatedWork W1968101187 @default.
• W4313552002 hasRelatedWork W1974496092 @default.
• W4313552002 hasRelatedWork W1980296501 @default.
• W4313552002 hasRelatedWork W2169073827 @default.
• W4313552002 hasRelatedWork W2223206920 @default.
• W4313552002 hasRelatedWork W2346624621 @default.
• W4313552002 hasRelatedWork W2395342967 @default.
• W4313552002 hasRelatedWork W4250292491 @default.
• W4313552002 hasRelatedWork W4251979249 @default.
• W4313552002 hasVolume "23" @default.
• W4313552002 isParatext "false" @default.

• next