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- W4313554712 abstract "Here, we report the development of a novel in vitro digestion system that mimics the digestive process in fish and uses the RTgutGC cell line, a model of the rainbow trout (Oncorhynchus mykiss) intestine, to study the bioavailability, bioreactivity, and toxicity of Zn added as an inorganic (ZnSO4) and organic zinc compound (Zn-Bioplex). Our results showed that, before digestion, Zn-Bioplex was more bioavailable than ZnSO4. However, after in vitro digestion, ZnSO4 and Zn-Bioplex accumulated and were transported equally across the RTgutGC cell epithelium. However, Zn-Bioplex was shown to be less bioreactive than ZnSO4, suggesting that peptide complexation might reduce the intracellular release of zinc as shown by a reduced induction of metallothionein and ZnT1 mRNA levels. This novel method shows promise as a standardized in vitro fish digestion model to study the interactions occurring at the lumen of the intestine and the bioavailability and toxicity of digested compounds in the fish gut." @default.
- W4313554712 created "2023-01-06" @default.
- W4313554712 creator A5014343144 @default.
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- W4313554712 date "2023-01-05" @default.
- W4313554712 modified "2023-10-17" @default.
- W4313554712 title "Development of an <i>in Vitro</i> Digestion System to Study the Bioavailability and Bioreactivity of Zinc Sulfate and Zn-Bioplex in Fish Using the RTgutGC Cell Line" @default.
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- W4313554712 doi "https://doi.org/10.1021/acsfoodscitech.2c00307" @default.
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