Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313555965> ?p ?o ?g. }
- W4313555965 abstract "SUMMARY The functionality of essential metabolic processes in chloroplasts depends on a balanced integration of nuclear-and chloroplast-encoded polypeptides into the plastid’s proteome. The chloroplast chaperonin machinery is an essential player in chloroplast protein folding with a more intricate structure and subunit composition compared to the orthologous GroEL/ES chaperonin of Escherichia coli . However, its exact role in chloroplasts remains obscure, mainly because of a very limited knowledge about the folded substrates. We employed the competition immunoprecipitation method for the identification of the chaperonin’s substrates in Chlamydomonas reinhardtii . Co-immunoprecipitation of the target complex in the presence of increasing amounts of isotope-labelled competitor epitope and subsequent mass spectrometry analysis specifically allowed to distinguish true interactors from unspecifically co-precipitated proteins. Besides known substrates such as RbcL, we revealed numerous new substrates with high confidence. Identified substrate proteins differ from bulk chloroplast proteins by a higher content of beta-sheets, lower alpha-helical content and increased aggregation propensity. Immunoprecipitations performed with a subunit of the co-chaperonin lid revealed the ClpP protease as a specific partner complex, with altered interactions during heat stress, pointing to a close collaboration of these machineries to maintain protein homeostasis in the chloroplast." @default.
- W4313555965 created "2023-01-06" @default.
- W4313555965 creator A5019096353 @default.
- W4313555965 creator A5026003971 @default.
- W4313555965 creator A5049783868 @default.
- W4313555965 creator A5066522492 @default.
- W4313555965 creator A5074206296 @default.
- W4313555965 creator A5087310264 @default.
- W4313555965 creator A5091469086 @default.
- W4313555965 date "2023-01-05" @default.
- W4313555965 modified "2023-10-13" @default.
- W4313555965 title "Competition co-immunoprecipitation reveals interactors of the chloroplast CPN60 chaperonin machinery" @default.
- W4313555965 cites W1533808701 @default.
- W4313555965 cites W1599232010 @default.
- W4313555965 cites W1686243412 @default.
- W4313555965 cites W177193660 @default.
- W4313555965 cites W1964895626 @default.
- W4313555965 cites W1966334222 @default.
- W4313555965 cites W1971824923 @default.
- W4313555965 cites W1976587162 @default.
- W4313555965 cites W1980656534 @default.
- W4313555965 cites W1982079790 @default.
- W4313555965 cites W1994583786 @default.
- W4313555965 cites W2001153067 @default.
- W4313555965 cites W2005369928 @default.
- W4313555965 cites W2006594997 @default.
- W4313555965 cites W2013083078 @default.
- W4313555965 cites W2017839625 @default.
- W4313555965 cites W2029086579 @default.
- W4313555965 cites W2035292024 @default.
- W4313555965 cites W2038072396 @default.
- W4313555965 cites W2038761308 @default.
- W4313555965 cites W2041506715 @default.
- W4313555965 cites W2045777307 @default.
- W4313555965 cites W2047264659 @default.
- W4313555965 cites W2052815574 @default.
- W4313555965 cites W2055198406 @default.
- W4313555965 cites W2062996895 @default.
- W4313555965 cites W2086272648 @default.
- W4313555965 cites W2088998421 @default.
- W4313555965 cites W2092850779 @default.
- W4313555965 cites W2096622242 @default.
- W4313555965 cites W2097169575 @default.
- W4313555965 cites W2098665638 @default.
- W4313555965 cites W2106765984 @default.
- W4313555965 cites W2119361724 @default.
- W4313555965 cites W2122326704 @default.
- W4313555965 cites W2122623449 @default.
- W4313555965 cites W2129214482 @default.
- W4313555965 cites W2138749342 @default.
- W4313555965 cites W2145487893 @default.
- W4313555965 cites W2147289825 @default.
- W4313555965 cites W2148636009 @default.
- W4313555965 cites W2153574614 @default.
- W4313555965 cites W2160084211 @default.
- W4313555965 cites W2167491998 @default.
- W4313555965 cites W2214850930 @default.
- W4313555965 cites W2238452763 @default.
- W4313555965 cites W2340925051 @default.
- W4313555965 cites W2473711714 @default.
- W4313555965 cites W2750873278 @default.
- W4313555965 cites W2752143406 @default.
- W4313555965 cites W2775683081 @default.
- W4313555965 cites W2792031579 @default.
- W4313555965 cites W2884277554 @default.
- W4313555965 cites W2888898106 @default.
- W4313555965 cites W2902966703 @default.
- W4313555965 cites W2909996959 @default.
- W4313555965 cites W2949362552 @default.
- W4313555965 cites W2975161022 @default.
- W4313555965 cites W3005296459 @default.
- W4313555965 cites W3008974935 @default.
- W4313555965 cites W3033612824 @default.
- W4313555965 cites W3037788827 @default.
- W4313555965 cites W3094471368 @default.
- W4313555965 cites W3112570663 @default.
- W4313555965 cites W3131597813 @default.
- W4313555965 cites W3212053275 @default.
- W4313555965 cites W4200416892 @default.
- W4313555965 cites W4205764878 @default.
- W4313555965 cites W4231432280 @default.
- W4313555965 doi "https://doi.org/10.1101/2023.01.05.522938" @default.
- W4313555965 hasPublicationYear "2023" @default.
- W4313555965 type Work @default.
- W4313555965 citedByCount "0" @default.
- W4313555965 crossrefType "posted-content" @default.
- W4313555965 hasAuthorship W4313555965A5019096353 @default.
- W4313555965 hasAuthorship W4313555965A5026003971 @default.
- W4313555965 hasAuthorship W4313555965A5049783868 @default.
- W4313555965 hasAuthorship W4313555965A5066522492 @default.
- W4313555965 hasAuthorship W4313555965A5074206296 @default.
- W4313555965 hasAuthorship W4313555965A5087310264 @default.
- W4313555965 hasAuthorship W4313555965A5091469086 @default.
- W4313555965 hasBestOaLocation W43135559651 @default.
- W4313555965 hasConcept C104292427 @default.
- W4313555965 hasConcept C104317684 @default.
- W4313555965 hasConcept C104397665 @default.
- W4313555965 hasConcept C143065580 @default.
- W4313555965 hasConcept C204328495 @default.
- W4313555965 hasConcept C2777727519 @default.